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| Product Name | Acetate Assay Reagent |
| Catalog No. | CMTR-HMM-0052 |
| Description | Acetate is an important component of biosynthesis. In organisms, the acetylation/deacetylation of proteins is crucial for their post-translational regulation of function. Acetyl-CoA, when bound to coenzyme A, is essential for the metabolism of carbohydrates and fats. |
| Application | This product is used to quantify acetate levels in biological samples. |
| Principle | In the presence of an acetate enzyme mixture and an acetate substrate mixture, free acetate is converted into an intermediate. This intermediate reduces the probe to a colored product with strong absorbance at OD = 450 nm. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 50 min |
| Sample Type | Adherent cells, Serum, Plasma, Suspension cells, Tissue |
| Detection Limit | 20 µM |
| Procedure | 1. Add samples and standards to wells. 2. Add reaction mixture and incubate for 40 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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