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| Product Name | 4x Multiplex PCR Master Mix, Low-Background Version (1-2000-Plex Pathogen tNGS) |
| Catalog No. | REKI-0107 |
| Description | A 4x concentrated master mix developed for two-round multiplex amplification in amplicon-based library construction. Combined hot-start chemistry and a tuned buffer system scale from singleplex up to 2000-plex panels while keeping primer-dimer and background signatures low. Amplicons across a 25 to 75 percent GC window amplify with high uniformity, specificity and sensitivity, and 30 to 500 primer pairs of differing amplicon sizes work in the same reaction, with headroom into the several-thousand-plex range for amplicon capture. The mix accepts reverse-transcribed cDNA directly, so RNA viruses join DNA pathogens in a single workflow without an intermediate cleanup. |
| Intended Use | Pathogen targeted NGS panel construction, tNGS amplicon library preparation, co-detection of DNA and RNA pathogens in syndromic panels. |
| Principle / Technology | Two rounds of hot-start multiplex PCR build and enrich amplicon libraries; target-specific primers define the panel while the master mix supplies enzyme, buffer and dNTPs. |
| Performance Range / Specifications | Panel depth 1 to 2000-plex; 30 to 500 amplicon pairs per reaction; amplicon GC coverage 25 to 75 percent; high uniformity, specificity and sensitivity. |
| Specificity | Reverse-transcribed samples enter multiplex PCR directly without purification. |
| Reaction Conditions / Protocol | Combine template, panel primers and 4x master mix, run the two-round multiplex programme, then proceed to library finishing. |
| Components / Formulation | Hot-start multiplex polymerase in 4x premix format, optimised reaction buffer, dNTPs. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 2 years |
| Package Specifications | 24 T, 48 T, 96 T, 1000 T |
| Product Form | Liquid premix |
| Key Features | Low-background chemistry protects rare-variant calls; broad GC tolerance; direct RT compatibility simplifies RNA virus workflows. |
| Concentration | 4x premix |
| Compatibility | Pathogen tNGS workflows on short-read sequencing platforms |
| Readouts / Downstream Assays | Multiplex amplicon libraries for syndromic pathogen sequencing panels |
For research use only, not for clinical use.
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