- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | 4x Multiplex PCR Master Mix 2.0 (1-2000-Plex Amplicon Library Construction) |
| Catalog No. | REKI-0111 |
| Description | A second-generation 4x multiplex master mix for two-round amplicon library construction in pathogen sequencing. Hot-start chemistry and a refined buffer carry panels of 1 to 2000-plex with high uniformity, specificity and sensitivity, and amplicons spanning a 25 to 75 percent GC window amplify side by side. Reactions accept 30 to 500 primer pairs of mixed amplicon sizes, scale into several-thousand-plex capture, and take reverse-transcribed cDNA directly so DNA and RNA pathogens share one workflow. |
| Intended Use | Two-round multiplex amplicon library construction, DNA and RNA pathogen co-detection, targeted sequencing panel enrichment. |
| Principle / Technology | Hot-start multiplex PCR with panel-specific primers builds amplicon libraries; the master mix supplies polymerase, buffer and dNTPs in 4x format. |
| Performance Range / Specifications | Panel depth 1 to 2000-plex; 30 to 500 primer pairs per reaction; GC coverage 25 to 75 percent; high uniformity, specificity and sensitivity. |
| Specificity | Reverse transcription products enter amplification without purification; hot-start chemistry suppresses non-specific priming. |
| Reaction Conditions / Protocol | Combine template, panel primers and master mix, run the two-round programme, then proceed to library finishing. |
| Components / Formulation | Hot-start multiplex polymerase premix, optimised buffer, dNTPs. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 24 T, 48 T, 100 T |
| Product Form | Liquid premix |
| Key Features | Second-generation buffer widens GC tolerance; direct RT compatibility; scalable from small panels to thousands of targets. |
| Concentration | 4x premix |
| Compatibility | Pathogen tNGS and amplicon sequencing workflows |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
