2x Low-Background Multiplex PCR Master Mix (GC 25-70 Percent, High Uniformity)

Name: 2x Low-Background Multiplex PCR Master Mix (GC 25-70 Percent, High Uniformity)
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Cat.No: REKI-0115 Datasheet

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Product Name	2x Low-Background Multiplex PCR Master Mix (GC 25-70 Percent, High Uniformity)
Catalog No.	REKI-0115
Description	A 2x premix built from a hot-start multiplex polymerase formulation and a dedicated amplification buffer. The chemistry spans templates from roughly 25 to 70 percent GC, multiplex reactions stay uniform, specific and sensitive, and gel verification of up to 20 amplicon pairs extends to enrichment of several hundred targets in sequencing panel workflows. Cold-chain-friendly storage at 2 to 8°Celsius and bulk packaging options suit both clinical-adjacent research labs and high-throughput operations.
Intended Use	Multiplex target enrichment for sequencing panels, multiplex amplicon library construction, GC-challenging template amplification.
Principle / Technology	Hot-start multiplex PCR concentrates target amplicons while the low-background formulation suppresses primer-dimer and off-target signals.
Performance Range / Specifications	GC compatibility around 25 to 70 percent; high uniformity, specificity and sensitivity; supports several-hundred-plex enrichment.
Specificity	Hot-start formulation; low background protects downstream sequencing signal-to-noise.
Reaction Conditions / Protocol	Combine template, panel primers and 2x premix, then run the recommended multiplex programme.
Components / Formulation	Hot-start multiplex polymerase, 2x amplification buffer, dNTPs.
Storage Conditions	2 to 8 °C
Shelf Life	2 years
Package Specifications	1 mL, 5 x 1 mL, 50 mL, 250 mL, 500 mL
Product Form	Liquid premix
Key Features	Warm-storage format removes freezer dependency; bulk sizes for high-throughput panels; broad GC window.
Concentration	2x premix
Compatibility	Targeted sequencing and multiplex PCR workflows

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For research use only, not for clinical use.