- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | 2x High-Fidelity Library Amplification Mix with Dual-Antibody Hot Start |
| Catalog No. | REKI-0112 |
| Description | A ready-to-use 2x amplification premix combining a high-fidelity DNA polymerase, dNTPs and a buffer tuned for sequencing library PCR. Two monoclonal antibodies block both the polymerase and its 3-prime-to-5-prime exonuclease activity at room temperature, enabling genuine hot-start specificity and letting primers be pre-mixed into the solution for extended bench storage. A dedicated protective agent keeps activity stable through repeated freeze-thaw cycles, protecting lot performance in busy sequencing labs. |
| Intended Use | PCR enrichment of sequencing libraries, hot-start amplification of NGS adapters-ligated fragments, workflows requiring premixed primers and room-temperature handling. |
| Principle / Technology | Antibody-bound polymerase stays inactive until thermal denaturation releases it, preventing primer extension and exonucleolytic adapter damage before cycling begins. |
| Performance Range / Specifications | High sensitivity, specificity, fidelity and library yield; premix tolerates repeated freeze-thaw without activity loss. |
| Specificity | Dual-antibody hot start inhibits both polymerase and proofreading exonuclease at room temperature. |
| Reaction Conditions / Protocol | Add primers, template and the 2x premix, then run the recommended library amplification programme. |
| Components / Formulation | High-fidelity DNA polymerase with antibody hot start, dNTPs, protective agent, optimised buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 24 T, 96 T |
| Product Form | Liquid premix |
| Key Features | Room-temperature primer pre-mixing; freeze-thaw stabilised; high library conversion in sequencing PCR. |
| Concentration | 2x premix |
| Compatibility | Sequencing library amplification workflows on short-read platforms |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
