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| Product Name | 1152-Plex Unique Dual Index Primer Plates for Illumina Libraries |
| Catalog No. | NG-QCY-0052 |
| Description | A twelve-set plate collection providing universal partial adapters and 1,152 unique dual-index primer combinations for multiplex Illumina-compatible DNA library construction. |
| Intended Use | DNA library preparation, high-throughput sequencing, Illumina sequencing and automated library construction. |
| Principle / Technology | A universal partial adapter is completed during PCR with independently paired i5 and i7 index primers to reduce index hopping and misassignment. |
| Detection Method | High-throughput sequencing. |
| Sample Type | T-A ligation-based DNA libraries. |
| Performance Range / Specifications | Twelve 96-well sets provide 1,152 unique dual-index primer combinations; 96 reactions per set. |
| Specificity | Independent dual indexes support reliable sample demultiplexing in high-complexity pools. |
| Reaction Conditions / Protocol | Ligate the compatible universal partial adapter, then amplify each library with the assigned dual-index primer pair. |
| Components / Formulation | Universal partial adapter and twelve 96-well unique dual-index primer plates. |
| Package Specifications | 96 reactions per set; 12 sets available. |
| Product Form | Plate-format adapter and index-primer system. |
| Quality Control | Adapters and index primers are functionally validated for stable and reproducible library construction. |
| Key Features | 1,152 index combinations; automation-ready plate format; pierceable seals; reduced index hopping; broad T-A ligation compatibility. |
| Compatibility | Illumina sequencing platforms and compatible T-A ligation library preparation kits. |
| Application Notes / Precautions | Select balanced index combinations for the sequencing instrument and pooling design. |
| Batch-to-Batch Consistency | Library yield and index performance are controlled across wells and batches. |
For research use only, not for clinical use.
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