1152-Plex Unique Dual Index Primer Plates for Illumina Libraries
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1152-Plex Unique Dual Index Primer Plates for Illumina Libraries

Cat.No: NG-QCY-0052 Datasheet

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Product Name 1152-Plex Unique Dual Index Primer Plates for Illumina Libraries
Catalog No. NG-QCY-0052
Description A twelve-set plate collection providing universal partial adapters and 1,152 unique dual-index primer combinations for multiplex Illumina-compatible DNA library construction.
Intended Use DNA library preparation, high-throughput sequencing, Illumina sequencing and automated library construction.
Principle / Technology A universal partial adapter is completed during PCR with independently paired i5 and i7 index primers to reduce index hopping and misassignment.
Detection Method High-throughput sequencing.
Sample Type T-A ligation-based DNA libraries.
Performance Range / Specifications Twelve 96-well sets provide 1,152 unique dual-index primer combinations; 96 reactions per set.
Specificity Independent dual indexes support reliable sample demultiplexing in high-complexity pools.
Reaction Conditions / Protocol Ligate the compatible universal partial adapter, then amplify each library with the assigned dual-index primer pair.
Components / Formulation Universal partial adapter and twelve 96-well unique dual-index primer plates.
Package Specifications 96 reactions per set; 12 sets available.
Product Form Plate-format adapter and index-primer system.
Quality Control Adapters and index primers are functionally validated for stable and reproducible library construction.
Key Features 1,152 index combinations; automation-ready plate format; pierceable seals; reduced index hopping; broad T-A ligation compatibility.
Compatibility Illumina sequencing platforms and compatible T-A ligation library preparation kits.
Application Notes / Precautions Select balanced index combinations for the sequencing instrument and pooling design.
Batch-to-Batch Consistency Library yield and index performance are controlled across wells and batches.

For research use only, not for clinical use.

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