1-Minute Sample Release Reagent for Extraction-Free PCR and LAMP
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1-Minute Sample Release Reagent for Extraction-Free PCR and LAMP

Cat.No: ATR-SPS-0092 Datasheet

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Product Name 1-Minute Sample Release Reagent for Extraction-Free PCR and LAMP
Catalog No. ATR-SPS-0092
Description Alta DiagnoTech sample release reagent is an ambient-stable direct-amplification lysis solution for molecular point-of-care workflows.
Intended Use For rapid preparation of nasal or throat swab samples for real-time PCR, RT-PCR or fluorescent LAMP without conventional nucleic-acid extraction.
Sample Type Nasal swabs and throat swabs
Performance Range / Specifications Batch consistency was evaluated by adding one nasal swab and 1,000 copies of a pseudovirus target to 800 µL release reagent; quantitative PCR amplification curves were similar between batches, with ΔCt within ±0.5 Ct.
Reaction Conditions / Protocol Add 800 µL reagent to a 1.5 mL collection tube. Collect the nasal or throat swab, place it immediately into the tube and rotate the swab head in the liquid for at least 30 seconds. Incubate for 1 minute at 20-25°C. Up to 10 µL lysate can be added to a 25 µL RT-PCR or RT-LAMP reaction.
Storage Conditions Store at ambient temperature.
Package Specifications Available in 1 mL, 8 mL, 100 mL and 1,000 mL formats.
Product Form Liquid sample-release reagent
Shipping Conditions Ship at ambient temperature.
Compatibility Compatible with buffered PCR, RT-PCR and fluorescent LAMP systems. Not compatible with unbuffered colorimetric LAMP because the lysate is acidic.
Application Notes / Precautions Reagent volume may be reduced to 400 µL per swab if required. Proceed to amplification as soon as possible after treatment, preferably within 1 hour.
Product Type Direct-amplification sample lysis reagent
Function / Efficacy Releases nucleic acid from swab samples for direct amplification, shortening sample preparation time.

For research use only, not for clinical use.

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