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| Product Name | Universal Transfection Reagent |
| Catalog No. | RESK-0226 |
| Description | Universal Transfection Reagent is a versatile, convenient, and highly efficient liposome-based transfection reagent developed with advanced nanotechnology. It is suitable for the transfection of DNA, RNA, and oligonucleotides and delivers high transfection efficiency even in hard-to-transfect cells. Its unique formulation allows it to be added directly to the culture medium, and the presence of serum does not affect transfection efficiency, thereby minimizing cell damage caused by serum removal. After transfection, there is no need to remove the nucleic acid–transfection reagent complexes or replace the medium; if desired, fresh medium can be added 4–6 hours post-transfection depending on the nutritional status of the cells. |
| Intended Use | Suitable for transient transfection and generation of stably transfected cell lines. Low cytotoxicity: transfected cells retain excellent viability. Broad applicability: covers both common cell lines and hard-to-transfect cells. Easy to use: compatible with serum-containing medium; no medium change is required before or after transfection. |
| Principle / Technology | Liposome-based transfection reagent developed using advanced nanotechnology. It forms nucleic acid–transfection reagent complexes that can be added directly to the culture medium; serum does not interfere with transfection efficiency. |
| Sample Type | DNA, RNA, and oligonucleotides; common and hard-to-transfect mammalian cells (e.g., 293T and MCF-7 cells transfected with an EGFP expression plasmid). |
| Reaction Conditions / Protocol | Add the nucleic acid–transfection reagent complex directly to the culture medium. No medium change is required after transfection; fresh medium may optionally be added 4–6 hours post-transfection depending on cell nutritional status. |
| Components / Formulation | Liposome-based transfection reagent formulated with advanced nanotechnology. |
| Storage Conditions | Store at 2–8 °C. DO NOT freeze. |
| Shelf Life | 1 year when stored at 2–8 °C. |
| Package Specifications | 0.5 mL 1 mL 5 × 1 mL |
| Product Form | Liquid |
| Shipping Conditions | Shipped with wet ice. |
| Expiration Date / Stability | Stable for 1 year at 2–8 °C; do not freeze. |
| Compatibility | Compatible with serum-containing culture media; suitable for DNA, RNA, and oligonucleotide transfection in a broad range of cell types, including hard-to-transfect cells. |
| Application Notes / Precautions | Do not freeze the product. Serum does not need to be removed from the culture medium. No medium change is required after transfection unless cell nutrition status warrants it (4–6 hours post-transfection). |
For research use only, not for clinical use.
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