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| Product Name | Biodegradable Polymer DNA Transfection Reagent |
| Catalog No. | ATR-CTR-0031 |
| Description | A low-toxicity transfection reagent based on a biodegradable, activated dendritic polymer with uniform size and defined architecture for reproducible plasmid DNA delivery. |
| Intended Use | Plasmid DNA transfection of adherent and suspension mammalian cells. |
| Principle / Technology | A biodegradable cationic polymer forms complexes with DNA, promotes cellular uptake and then rapidly releases the nucleic acid while the carrier degrades. |
| Sample Type | Adherent and suspension mammalian cell cultures. |
| Performance Range / Specifications | Suggested optimization range: 1-3 µL reagent per µg DNA; for a 24-well plate, approximately 0.5-1 µg DNA per well may be evaluated. |
| Reaction Conditions / Protocol | Dilute reagent and DNA separately in PBS or serum-free medium, combine and incubate to form complexes, then add to complete culture medium. |
| Components / Formulation | Biodegradable polymer transfection reagent. |
| Storage Conditions | Store at 4-8°C. Do not freeze. |
| Package Specifications | 1 mL; 4 × 1 mL. |
| Product Form | Liquid polymer transfection reagent. |
| Key Features | Low cytotoxicity; reproducible performance; broad cell compatibility; serum and antibiotic tolerance; no routine medium change. |
| Compatibility | Complete medium with or without serum; antibiotics do not materially interfere with the stated workflow. |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio and cell density. Many cells perform well at 60-80% confluence, and high-purity sterile endotoxin-free DNA is recommended. |
| Batch-to-Batch Consistency | Uniform polymer size and defined architecture support reproducible transfection results. |
For research use only, not for clinical use.
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