Biodegradable Polymer DNA Transfection Reagent
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Biodegradable Polymer DNA Transfection Reagent

Cat.No: ATR-CTR-0031 Datasheet

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Product Name Biodegradable Polymer DNA Transfection Reagent
Catalog No. ATR-CTR-0031
Description A low-toxicity transfection reagent based on a biodegradable, activated dendritic polymer with uniform size and defined architecture for reproducible plasmid DNA delivery.
Intended Use Plasmid DNA transfection of adherent and suspension mammalian cells.
Principle / Technology A biodegradable cationic polymer forms complexes with DNA, promotes cellular uptake and then rapidly releases the nucleic acid while the carrier degrades.
Sample Type Adherent and suspension mammalian cell cultures.
Performance Range / Specifications Suggested optimization range: 1-3 µL reagent per µg DNA; for a 24-well plate, approximately 0.5-1 µg DNA per well may be evaluated.
Reaction Conditions / Protocol Dilute reagent and DNA separately in PBS or serum-free medium, combine and incubate to form complexes, then add to complete culture medium.
Components / Formulation Biodegradable polymer transfection reagent.
Storage Conditions Store at 4-8°C. Do not freeze.
Package Specifications 1 mL; 4 × 1 mL.
Product Form Liquid polymer transfection reagent.
Key Features Low cytotoxicity; reproducible performance; broad cell compatibility; serum and antibiotic tolerance; no routine medium change.
Compatibility Complete medium with or without serum; antibiotics do not materially interfere with the stated workflow.
Application Notes / Precautions Optimize reagent-to-DNA ratio and cell density. Many cells perform well at 60-80% confluence, and high-purity sterile endotoxin-free DNA is recommended.
Batch-to-Batch Consistency Uniform polymer size and defined architecture support reproducible transfection results.

For research use only, not for clinical use.

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