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| Product Name | Nucleofector Solution Kit for Primary Mammalian Cells |
| Catalog No. | ATR-CTR-0006 |
| Description | A cell type-specific nucleofection solution kit combining a specialized electroporation buffer with a pre-programmed device protocol, enabling efficient and reproducible delivery of nucleic acids directly into the nucleus of primary cells and difficult-to-transfect lines. |
| Intended Use | Transfection of primary human and mouse cells including T cells, B cells, monocytes, keratinocytes, neurons, and mesenchymal stem cells for functional genomics, immunotherapy research, and gene editing applications. |
| Principle / Technology | Proprietary Nucleofector solution combined with a cell type-specific electrical pulse program transiently permeabilizes both the cytoplasmic and nuclear membranes, delivering DNA directly to the nucleoplasm independent of cell division, a critical advantage for non-dividing primary cells. |
| Detection Method | Reporter gene expression kinetics (GFP visible within 2–4 hours indicating direct nuclear delivery), flow cytometric viability and transfection efficiency |
| Sample Type | Primary human T cells, B cells, monocytes, dendritic cells, NK cells, HUVEC, keratinocytes, neurons, MSCs, satellite cells |
| Performance Range / Specifications | Transfection efficiency 40–90% for primary cells; viability 50–80% depending on cell type; GFP expression detectable within 2–4 hours |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Resuspend 1–5 × 10^6 cells in 100 µL Nucleofector solution; add 1–5 µg DNA or 30–300 pmol siRNA; transfer to cuvette; run cell type-specific program; immediately add pre-warmed culture medium; transfer to culture. |
| Components / Formulation | Nucleofector solution (cell type specific), cuvettes, pipette tips, positive control pmaxGFP plasmid |
| Storage Conditions | 2–8°C; Nucleofector Solution protect from light |
| Shelf Life | 12 months |
| Package Specifications | Kit for 25 reactions, 100 reactions |
| Product Form | Liquid buffer with disposable cuvettes |
| Quality Control | Transfection efficiency and viability validation for specified cell type, sterility, endotoxin |
| Key Features | Cell type-specific optimization; direct nuclear delivery; effective for non-dividing cells; ready-to-use solutions; validated for primary cell applications; high viability |
For research use only, not for clinical use.
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