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| Product Name | Universal Topoisomerase-Mediated TA/Blunt Cloning Kit |
| Catalog No. | NATR-HMM-0168 |
| Description | A topoisomerase-mediated, low-background cloning system that accepts both A-tailed and blunt-ended PCR products without separate vector preparation. |
| Intended Use | TA cloning; blunt-end cloning; preservation and sequencing of DNA fragments; rapid subcloning; high-throughput cloning; library construction. |
| Principle / Technology | Topoisomerase-mediated DNA joining is combined with blunt-end conversion activity to insert A-tailed or blunt-ended PCR fragments into a ready-to-use cloning vector. |
| Sample Type | A-tailed or blunt-ended PCR products. |
| Performance Range / Specifications | Compatible with inserts from 150 bp to 10 kb; five-minute ligation at 25°C; supports both TA and blunt-end cloning. |
| Specificity | Designed for efficient recovery of correctly inserted recombinant clones with low background. |
| Reaction Conditions / Protocol | Combine the PCR fragment with the cloning system and incubate at 25°C for 5 minutes before transformation. |
| Components / Formulation | Topoisomerase-mediated TA/blunt cloning vector system with blunt-end conversion activity and supporting cloning reagents. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 20 reactions; 40 reactions. |
| Product Form | Topoisomerase-mediated cloning kit. |
| Quality Control | Performance has been evaluated with A-tailed and blunt-ended inserts of different lengths. |
| Key Features | Accepts TA and blunt-ended products; five-minute workflow; low background; broad insert-size range; suitable for rapid and high-throughput cloning. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with A-tailed and blunt-ended PCR products ranging from 150 bp to 10 kb. |
| Application Notes / Precautions | Use purified PCR products when nonspecific amplification products are present. Transformation should be performed with suitable cloning competent cells. |
For research use only, not for clinical use.
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