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| Product Name | High-Specificity miRNA SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0199 |
| Description | A blue, ready-to-use dye-based qPCR premix optimized for sensitive miRNA expression analysis and single-nucleotide discrimination. |
| Intended Use | Quantitative analysis of miRNA expression. |
| Principle / Technology | An antibody-mediated hot-start Taq polymerase remains tightly inhibited at 55°C and is combined with an optimized qPCR buffer, intercalating fluorescent dye and universal passive reference dye. |
| Detection Method | SYBR Green I intercalating-dye real-time PCR. |
| Sample Type | miRNA-derived cDNA prepared by stem-loop or poly(A)-tailing reverse transcription. |
| Performance Range / Specifications | 125 or 500 reactions; produces reliable standard curves across a broad quantitative range. |
| Sensitivity / LOD | Detects target miRNA from picogram-level total RNA inputs. |
| Specificity | Discriminates single-nucleotide differences with reported nonspecific recognition below 5%. |
| Reaction Conditions / Protocol | Combine the master mix with target-specific primers and miRNA-derived cDNA, then run a compatible dye-based qPCR program. |
| Components / Formulation | Blue miRNA qPCR master mix, universal stem-loop reverse primer and human/rat/mouse U6 reference-gene primer set. |
| Storage Conditions | Store at −30 to −15°C protected from light. After thawing, the master mix can be stored at 2–8°C protected from light for up to 6 months. |
| Package Specifications | 125 reactions; 500 reactions. |
| Product Form | Blue 2× miRNA dye-based qPCR master mix. |
| Quality Control | Sensitivity, quantitative linearity and single-nucleotide discrimination are evaluated by miRNA real-time PCR. |
| Key Features | Picogram-level sensitivity; single-nucleotide discrimination; universal passive reference dye; supplied reference-gene primers; visible blue formulation. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common real-time PCR instruments and cDNA generated by stem-loop or poly(A)-tailing miRNA reverse-transcription methods. |
For research use only, not for clinical use.
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