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| Product Name | One-Tube Fast Multiplex RT-qPCR Master Mix with Primer/Probe Premix Support |
| Catalog No. | NATR-HMM-0242 |
| Description | Alta DiagnoTech one-tube RT-qPCR master mix supports singleplex or multiplex quantitative amplification directly from RNA templates. |
| Intended Use | For singleplex or multiplex probe-based quantitative PCR using RNA templates. |
| Principle / Technology | Reverse transcription and real-time PCR take place sequentially in one tube. A UDG-dUTP system reduces carryover contamination from previous amplification products. |
| Detection Method | Probe-based real-time fluorescence detection |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 100-, 1,000- and 5,000-reaction formats. |
| Product Form | One-tube liquid RT-qPCR master mix |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Supports advance premixing of primers and probes; the prepared working solution can be stored at low temperature before sample nucleic acid is added. |
| Application Notes / Precautions | Use RNase-free consumables. Recommended primers are 18-30 bases long with 40-60% GC content and a melting temperature of 55-60°C; recommended amplicons are 70-200 bp. Recommended probes are 18-30 bases long with a melting temperature of 65-70°C and HPLC-grade or better purification. |
| Product Type | Fast one-step multiplex RT-qPCR master mix |
| Function / Efficacy | Simplifies RNA testing, supports fast cycling and helps limit aerosol-derived carryover contamination. |
For research use only, not for clinical use.
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