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| Product Name | 5′/3′ Rapid Amplification of cDNA Ends Kit |
| Catalog No. | NATR-HMM-0182 |
| Description | A rapid amplification of cDNA ends system that combines template-switching reverse transcription with high-fidelity PCR to recover unknown transcript termini. |
| Intended Use | 5′ RACE; 3′ RACE; transcript-end identification; full-length cDNA analysis. |
| Principle / Technology | A template-switching reverse transcriptase generates full-length cDNA, followed by high-fidelity amplification of the 5′ or 3′ end without a separate adapter-ligation step. |
| Detection Method | PCR amplification and downstream gel analysis or sequencing. |
| Sample Type | Purified RNA. |
| Performance Range / Specifications | 10 reactions; reverse transcription demonstrated with transcripts up to 15 kb and abundance as low as 0.9 RPKM; tested transcript GC range approximately 36–70%. |
| Reaction Conditions / Protocol | Prepare full-length cDNA with the template-switching reverse-transcription module, then amplify the target 5′ or 3′ end with a gene-specific primer and the supplied PCR system. |
| Components / Formulation | Template-switching reverse-transcription module and high-fidelity PCR amplification module. |
| Storage Conditions | Store Box 1 at −80 to −65°C and Box 2 at −30 to −15°C. |
| Package Specifications | 10 reactions. |
| Product Form | Two-module 5′/3′ RACE kit. |
| Key Features | No adapter ligation; long-transcript capability; low-abundance transcript compatibility; high-fidelity amplification; supports both 5′ and 3′ RACE. |
| Shipping Conditions | Ship on dry ice. |
| Compatibility | Compatible with transcripts spanning a broad length, abundance and GC-content range. |
| Application Notes / Precautions | For low-abundance targets, RNA input can be increased up to 4 µg. Use a gene-specific primer with a melting temperature of at least 65°C. For amplicons up to 3 kb, allow approximately 3 minutes of extension and add about 30 seconds per additional kilobase. |
For research use only, not for clinical use.
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