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| Product Name | Universal Rapid Multi-Site Mutagenesis Kit |
| Catalog No. | NATR-HMM-0165 |
| Description | An integrated high-fidelity amplification and seamless recombination system for introducing mutations at one to five noncontiguous sites in a target plasmid. |
| Intended Use | Single- to five-site plasmid mutagenesis, including substitutions, insertions and deletions. |
| Principle / Technology | Rapid high-fidelity plasmid amplification is followed by methylated-template digestion and homologous recombination-mediated plasmid recircularization. |
| Sample Type | Circular plasmid DNA templates up to at least 10 kb in demonstrated workflows. |
| Performance Range / Specifications | Supports simultaneous mutation at one to five noncontiguous sites; a 10 kb single-site mutation workflow can be completed in approximately 1.5 hours. |
| Specificity | Designed to provide high clone numbers and high positive mutation rates for single-, dual- and five-site workflows. |
| Reaction Conditions / Protocol | Amplify with mutagenic primers using a low amount of template, digest the methylated parental plasmid, and use the specific amplification product directly for recombination when appropriate. |
| Components / Formulation | Integrated rapid high-fidelity PCR amplification module and seamless recombination module. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10 reactions; 20 reactions. |
| Product Form | Rapid multi-site mutagenesis kit. |
| Key Features | One- to five-site mutagenesis; supports substitutions, insertions and deletions; low template requirement; rapid 10 kb workflow; high cloning success. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Compatible with DpnI-based parental-template removal and direct recombination of specific amplification products. |
For research use only, not for clinical use.
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