Universal Plant Total RNA Isolation Kit
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Universal Plant Total RNA Isolation Kit

Cat.No: DREK-0112 Datasheet

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Product Details Related Products
Product Name Universal Plant Total RNA Isolation Kit
Catalog No. DREK-0112
Description A rapid spin-column kit for total RNA purification from diverse plant tissues, including routine samples and tissues rich in polysaccharides or polyphenols. The workflow avoids phenol, chloroform and beta-mercaptoethanol.
Intended Use Plant total RNA preparation for RT-PCR, qPCR, RNA library construction, microarray analysis, Northern blotting, dot blotting and molecular cloning.
Principle / Technology Plant tissue lysis and impurity removal followed by silica-membrane RNA binding, washing and elution.
Detection Method Downstream RNA analysis.
Sample Type Routine plant tissues and plant tissues rich in polysaccharides or polyphenols.
Performance Range / Specifications Approximately 11-minute room-temperature workflow; 50 reactions.
Reaction Conditions / Protocol Grind plant tissue thoroughly, mix rapidly with lysis reagent, remove insoluble contaminants, bind RNA to the spin column, wash and elute.
Package Specifications 50 reactions.
Product Form Plant total RNA spin-column kit.
Key Features Eleven-minute workflow; broad plant compatibility; phenol-free, chloroform-free and beta-mercaptoethanol-free handling.
Compatibility RT-PCR, qPCR, RNA library preparation, microarray, blotting and cloning workflows.
Application Notes / Precautions Freeze freshly collected plant tissue rapidly and avoid repeated freeze-thaw cycles. Thorough cryogenic grinding and immediate mixing with lysis reagent are important for RNA recovery.

For research use only, not for clinical use.

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