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| Product Name | Universal Plant Total RNA Isolation Kit |
| Catalog No. | DREK-0112 |
| Description | A rapid spin-column kit for total RNA purification from diverse plant tissues, including routine samples and tissues rich in polysaccharides or polyphenols. The workflow avoids phenol, chloroform and beta-mercaptoethanol. |
| Intended Use | Plant total RNA preparation for RT-PCR, qPCR, RNA library construction, microarray analysis, Northern blotting, dot blotting and molecular cloning. |
| Principle / Technology | Plant tissue lysis and impurity removal followed by silica-membrane RNA binding, washing and elution. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Routine plant tissues and plant tissues rich in polysaccharides or polyphenols. |
| Performance Range / Specifications | Approximately 11-minute room-temperature workflow; 50 reactions. |
| Reaction Conditions / Protocol | Grind plant tissue thoroughly, mix rapidly with lysis reagent, remove insoluble contaminants, bind RNA to the spin column, wash and elute. |
| Package Specifications | 50 reactions. |
| Product Form | Plant total RNA spin-column kit. |
| Key Features | Eleven-minute workflow; broad plant compatibility; phenol-free, chloroform-free and beta-mercaptoethanol-free handling. |
| Compatibility | RT-PCR, qPCR, RNA library preparation, microarray, blotting and cloning workflows. |
| Application Notes / Precautions | Freeze freshly collected plant tissue rapidly and avoid repeated freeze-thaw cycles. Thorough cryogenic grinding and immediate mixing with lysis reagent are important for RNA recovery. |
For research use only, not for clinical use.
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