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| Product Name | Viral DNA/RNA Extraction Kit (Spin Column) |
| Catalog No. | RESK-0767 |
| Description | Spin column kit for extraction of viral nucleic acids from animal tissues, plasma, serum, lymph, culture supernatants, secretions, swabs, feces, milk, urine, and other sample types, with simultaneous recovery of both DNA and RNA from the sample. A novel silica matrix in the spin column, combined with an optimized lysis buffer formulation, maximizes recovery of high-purity viral nucleic acids. The purified viral DNA/RNA is of high purity and stable, reliable quality, suitable for downstream applications such as restriction enzyme digestion, PCR, reverse transcription, and RT-PCR. The spin column workflow delivers stable yields with low variation and consistent extraction efficiency across batches, and requires no phenol or chloroform. |
| Intended Use | Simultaneous extraction and purification of viral DNA and RNA from a broad range of biological samples for downstream applications including restriction digestion, PCR, reverse transcription, and RT-PCR. For research use only. |
| Principle / Technology | Silica-membrane spin column technology; selective nucleic acid binding to a silica matrix under chaotropic conditions, followed by wash and elution steps |
| Detection Method | Downstream analysis by PCR, reverse transcription, RT-PCR, or restriction enzyme digestion |
| Sample Type | Animal tissues, plasma, serum, lymph, culture supernatants, secretions, swabs, feces, milk, and urine; customer-validated samples include poultry tissues and respiratory secretions, chicken embryo allantoic fluid, feces, and cotton swabs |
| Performance Range / Specifications | Simultaneous co-extraction of viral DNA and RNA from a single sample High purity and yield for convenient downstream use Stable yields with low fluctuation and consistent batch-to-batch extraction efficiency No phenol or chloroform organic solvents required |
| Specificity | Novel silica matrix membrane with optimized lysis buffer formulation for maximal recovery of high-purity viral nucleic acids |
| Reaction Conditions / Protocol | 1. Lyse the sample with lysis buffer in the presence of Proteinase K. 2. Add binding buffer/ethanol and apply the lysate to the spin column. 3. Wash the column and elute purified viral DNA/RNA. Both DNA and RNA are co-purified; to obtain viral DNA only, treat the eluate with RNase afterward. |
| Components / Formulation | Viral nucleic acid lysis buffer (proprietary formulation) Proteinase K RNA/DNA-binding spin column with novel silica matrix membrane Wash buffers Elution buffer / nuclease-free water |
| Storage Conditions | Part I components: store at -25 to 15 °C; Part II components: store at room temperature. After opening, Proteinase K can be stored at 2-8 °C. |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Product Form | Spin column kit with ready-to-use buffer components |
| Quality Control | Consistent extraction efficiency verified across batches; high purity and yield confirmed for downstream PCR/RT-PCR applications |
| Purity | High-purity viral DNA/RNA suitable for restriction digestion, PCR, reverse transcription, and RT-PCR |
| Expiration Date / Stability | Stable for 2 years under recommended storage conditions; opened Proteinase K remains stable at 2-8 °C |
| Regulatory / Compliance | For research use only; not intended for diagnostic procedures |
| Recommended Buffer System | Proprietary chaotropic salt-based lysis/binding buffer system with ethanol-containing wash buffers and RNase-free elution water |
| Application Notes / Precautions | The kit co-extracts both DNA and RNA from the sample; if only viral DNA is required, digest the eluted nucleic acids with RNase. No phenol or chloroform organic solvents are used. |
| Batch-to-Batch Consistency | Spin column workflow delivers stable yields with minimal fluctuation; extraction efficiency is consistent across production batches |
For research use only, not for clinical use.
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