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| Product Name | Endotoxin-Free Plasmid DNA Maxi Extraction Kit |
| Catalog No. | DREK-0095 |
| Description | A large-scale plasmid DNA purification kit for bacterial cultures. Modified alkaline lysis and a selective endotoxin-removal step produce high-yield plasmid DNA suitable for transfection and routine molecular biology. |
| Intended Use | Large-scale plasmid DNA purification for restriction digestion, PCR, in vitro transcription, transformation, sequencing and cell transfection. |
| Principle / Technology | Modified SDS-alkaline lysis, selective endotoxin capture and silica-column purification. |
| Detection Method | Downstream DNA analysis, in vitro transcription and transfection performance. |
| Sample Type | 150-300 mL overnight bacterial culture. |
| Performance Range / Specifications | Plasmid DNA yield: 0.2-1.5 mg from high-copy cultures; extraction recovery: 80-90%; endotoxin level <0.1 EU/µg DNA; compatible with plasmids from approximately 3.0 kb to 21.5 kb in demonstrated testing. |
| Specificity | Selective plasmid recovery with efficient reduction of endotoxin, protein, genomic DNA and RNA contaminants. |
| Reaction Conditions / Protocol | Harvest the culture, resuspend and lyse the cells, neutralize and clarify, perform endotoxin removal, bind DNA to the purification column, wash and elute. |
| Components / Formulation | Alkaline lysis and neutralization reagents, endotoxin-removal reagent, RNase, wash and elution buffers, and large-capacity purification columns. |
| Storage Conditions | Store general components at 15-25°C. Store RNase at -30 to -15°C. The endotoxin-removal reagent may be stored at 2-8°C for one month or at -30 to -15°C for long-term storage. |
| Package Specifications | 10 reactions. |
| Product Form | Large-scale endotoxin-free plasmid DNA extraction kit. |
| Quality Control | Yield, DNA integrity, endotoxin level and transfection performance are assessed during release testing. |
| Key Features | 0.2-1.5 mg yield; 80-90% recovery; endotoxin <0.1 EU/µg; broad plasmid-size compatibility; transfection-ready DNA. |
| Shipping Conditions | Ship general components at room temperature and temperature-sensitive components at 0°C or below. |
| Compatibility | Cell transfection, in vitro transcription and routine molecular biology applications. |
| Application Notes / Precautions | For low-copy or large plasmids, increase culture input and proportional buffer volumes as directed; prewarm the elution buffer when appropriate. |
| Batch-to-Batch Consistency | Yield, purity and endotoxin-removal performance are controlled across production batches. |
For research use only, not for clinical use.
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