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| Product Name | Universal One-Step Seamless Cloning Kit V3 |
| Catalog No. | NATR-HMM-0160 |
| Description | A one-step seamless DNA cloning system using an optimized recombination mix for directional assembly of one to eleven fragments into a linearized vector. |
| Intended Use | Rapid cloning, high-throughput cloning, seamless cloning, vector construction and homologous recombination. |
| Principle / Technology | Recombinase-mediated assembly joins DNA fragments and linearized vectors through terminal homologous sequences. |
| Sample Type | Linearized vectors and one to eleven DNA fragments with compatible homologous ends. |
| Performance Range / Specifications | Supports one to eleven DNA fragments and is compatible with large vectors, long or high-/low-GC homologous regions and low DNA input. |
| Specificity | Positive clone rates above 95% were reported across approximately 30 evaluated systems. |
| Components / Formulation | Optimized 2× seamless recombination mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 20 reactions; 40 reactions. |
| Product Form | One-step seamless cloning kit. |
| Key Features | Assembly of up to eleven fragments; high positive clone rate; broad DNA-input tolerance; compatibility with difficult vectors and homologous regions; no routine insert purification or molar input calculation required. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Compatible with low-input DNA, long fragments, large vectors and homologous regions with high or low GC content. |
For research use only, not for clinical use.
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