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| Product Name | Universal One-Step Seamless Cloning Kit V2 |
| Catalog No. | NATR-HMM-0161 |
| Description | A one-step seamless cloning system optimized for efficient homologous assembly of one to five DNA fragments into a linearized vector. |
| Intended Use | Rapid cloning, high-throughput cloning, seamless cloning, vector construction and homologous recombination. |
| Principle / Technology | An optimized recombination system joins DNA fragments and vector ends through homologous terminal sequences. |
| Sample Type | Linearized vectors and one to five DNA fragments, including unpurified amplification products. |
| Performance Range / Specifications | Supports one to five fragments; demonstrated with 35 bp homology arms and homology-arm GC contents of 6.7% and 86.7%. |
| Specificity | Positive clone rates above 90% were reported across evaluated systems. |
| Reaction Conditions / Protocol | Perform recombination at 50°C for 5–30 minutes before transformation. |
| Components / Formulation | Optimized 2× seamless recombination mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 20 reactions; 40 reactions. |
| Product Form | One-step seamless cloning kit. |
| Key Features | One- to five-fragment assembly; rapid reaction; broad homology-arm length and GC compatibility; accepts unpurified products; high clone number and positive rate. |
| Compatibility | Compatible with long homology arms, high- or low-GC homology arms and unpurified DNA systems. |
For research use only, not for clinical use.
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