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Universal Multiplex Probe qPCR Mix with UDG

Cat.No: NATR-HMM-0196 Datasheet

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Product Name Universal Multiplex Probe qPCR Mix with UDG
Catalog No. NATR-HMM-0196
Description A universal multiplex probe qPCR mix with dUTP/UDG carryover control for sensitive DNA-target detection across diverse assay conditions.
Intended Use Gene-expression analysis; multiplex DNA quantification; DNA-virus and other pathogen detection.
Principle / Technology An antibody-mediated hot-start polymerase and optimized buffer support multiplex probe amplification, while the dUTP/UDG system reduces carryover contamination.
Detection Method Multiplex fluorescent probe-based real-time PCR.
Sample Type DNA templates, including DNA-virus targets.
Performance Range / Specifications 100, 500 or 2,500 reactions; supports fast cycling, multiplex detection and broad template, GC-content and primer-melting-temperature conditions.
Sensitivity / LOD Designed for sensitive detection of low-template targets.
Specificity Probe-based detection and hot-start polymerase control support specific amplification with well-defined sigmoidal curves.
Reaction Conditions / Protocol Add primers, probes and DNA template to the 2× mix and run a compatible standard or fast multiplex qPCR program.
Components / Formulation 2× multiplex probe qPCR mix containing antibody-mediated hot-start DNA polymerase, optimized buffer, dUTP/UDG system and passive-reference dye formulation.
Storage Conditions Store at −30 to −15°C.
Package Specifications 100 reactions; 500 reactions; 2,500 reactions.
Product Form 2× universal multiplex probe qPCR mix with UDG.
Key Features Low-template sensitivity; multiplex capability; fast-program compatibility; broad template tolerance; carryover contamination control.
Shipping Conditions Ship at or below 0°C.
Compatibility Passive-reference formulation 1 is suitable for instruments such as ABI 7900HT, 7300 and StepOnePlus; formulation 2 is suitable for ABI 7500, 7500 Fast and Stratagene Mx3000P; no passive reference is required for compatible Roche and Bio-Rad instruments.

For research use only, not for clinical use.

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