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| Product Name | Universal High-Sensitivity SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0191 |
| Description | A universal high-sensitivity SYBR qPCR master mix for reproducible nucleic acid quantification across a wide range of real-time PCR platforms. |
| Intended Use | Relative gene-expression analysis; absolute quantification; sensitive DNA and cDNA detection. |
| Principle / Technology | An antibody-mediated hot-start polymerase, optimized buffer, specificity enhancer, intercalating dye and universal passive-reference system support efficient fluorescence-based amplification. |
| Detection Method | Intercalating-dye real-time PCR. |
| Sample Type | DNA or cDNA templates. |
| Performance Range / Specifications | 500 or 2,500 reactions at 20 µL per reaction; compatible with standard and fast qPCR programs. |
| Sensitivity / LOD | Supports detection of single-digit template copies under suitable assay conditions. |
| Specificity | Hot-start amplification and a specificity-enhancing buffer reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Add primers and template to the master mix and run a compatible standard or fast real-time PCR program. |
| Components / Formulation | qPCR master mix containing antibody-mediated hot-start DNA polymerase, intercalating dye, optimized buffer, specificity enhancer and universal passive-reference dye. |
| Storage Conditions | Store at −20°C protected from light. |
| Package Specifications | 500 reactions (20 µL per reaction); 2,500 reactions (20 µL per reaction). |
| Product Form | Universal high-sensitivity 2× SYBR qPCR master mix. |
| Key Features | Single-digit-copy sensitivity; high specificity; universal passive-reference system; standard and fast cycling compatibility. |
| Expiration Date / Stability | After thawing, store at 4°C protected from light for up to 6 months. The mix remains stable through 30 freeze–thaw cycles, and a complete reaction mixture remains stable for up to 6 hours at 4°C. |
| Compatibility | Compatible with common real-time PCR instruments using or not using passive-reference normalization. |
For research use only, not for clinical use.
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