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| Product Name | Universal High-Sensitivity SYBR qPCR Master Mix for Low-Abundance Targets |
| Catalog No. | NATR-HMM-0189 |
| Description | A high-fluorescence universal SYBR qPCR master mix optimized for low-abundance targets and broad real-time PCR platform compatibility. |
| Intended Use | Sensitive relative or absolute quantification of low-abundance DNA and cDNA targets. |
| Principle / Technology | An antibody-mediated hot-start DNA polymerase, optimized buffer and specificity enhancer support efficient intercalating-dye qPCR with universal passive-reference compatibility. |
| Detection Method | Intercalating-dye real-time PCR. |
| Sample Type | DNA or cDNA templates, particularly low-copy targets. |
| Performance Range / Specifications | 500 or 2,500 reactions at 20 µL per reaction; detection of approximately 1.75 plasmid copies was demonstrated with a 75% positive rate in the evaluated assay. |
| Sensitivity / LOD | Designed for single-digit-copy and very low-abundance target detection. |
| Specificity | Hot-start amplification and a specificity-enhancing buffer help minimize nonspecific products. |
| Reaction Conditions / Protocol | Add primers and template to the master mix and run a compatible standard or fast real-time PCR program. |
| Components / Formulation | qPCR master mix containing antibody-mediated hot-start DNA polymerase, intercalating dye, optimized buffer, specificity enhancer and universal passive-reference dye. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 500 reactions (20 µL per reaction); 2,500 reactions (20 µL per reaction). |
| Product Form | Universal high-sensitivity 2× SYBR qPCR master mix. |
| Quality Control | Low-copy detection, amplification efficiency and assay reproducibility are evaluated by real-time PCR. |
| Key Features | High fluorescence output; very low-copy sensitivity; strong specificity; universal passive-reference compatibility; suitable for standard and fast programs. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Stable for up to 24 months at −20°C, 9 days at 37°C, 1 month at 25°C and 30 freeze–thaw cycles under the evaluated conditions. After thawing, store at 2–8°C protected from light for up to 6 months. |
| Compatibility | Compatible with common real-time PCR instruments using or not using passive-reference normalization. |
For research use only, not for clinical use.
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