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| Product Name | Universal Heat-Labile UDG Probe qPCR Master Mix |
| Catalog No. | NATR-HMM-0193 |
| Description | A universal probe qPCR master mix combining a chemically modified hot-start polymerase with a dUTP and heat-labile UDG carryover-control system. |
| Intended Use | Probe-based gene-expression analysis and sensitive nucleic acid quantification. |
| Principle / Technology | Heat-labile UDG removes uracil-containing carryover products before amplification, while a chemically modified hot-start polymerase drives sequence-specific probe qPCR. |
| Detection Method | Fluorescent probe-based real-time PCR. |
| Sample Type | DNA or cDNA templates. |
| Performance Range / Specifications | 500 or 2,500 reactions at 20 µL per reaction; amplification efficiency up to approximately 99% under the evaluated conditions. |
| Sensitivity / LOD | Supports single-copy template detection under suitable assay conditions. |
| Specificity | Probe-based detection and hot-start amplification support sequence-specific quantification. |
| Reaction Conditions / Protocol | Add primers, probe and template to the master mix and run a compatible probe qPCR program that includes activation of the carryover-control system. |
| Components / Formulation | Probe qPCR master mix containing chemically modified hot-start DNA polymerase, dUTP, heat-labile UDG and universal passive-reference dye. |
| Storage Conditions | Store at −20°C protected from light. |
| Package Specifications | 500 reactions (20 µL per reaction); 2,500 reactions (20 µL per reaction). |
| Product Form | Universal 2× probe qPCR master mix with heat-labile UDG. |
| Key Features | Carryover contamination control; single-copy sensitivity; high amplification efficiency; universal passive-reference compatibility. |
| Expiration Date / Stability | Stable for up to 3 weeks at 4°C and through 30 freeze–thaw cycles under the evaluated conditions. A complete reaction mixture remains stable for up to 6 hours at 4°C. |
| Compatibility | Compatible with common probe-based real-time PCR instruments. |
For research use only, not for clinical use.
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