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| Product Name | Universal Cell and Tissue Total RNA Isolation Kit |
| Catalog No. | DREK-0108 |
| Description | A universal silica-column kit for total RNA isolation from cultured cells and animal tissues, including protein-rich, fibrous and fatty samples. Enhanced lysis and optimized washing reduce protein, genomic DNA and other contaminants. |
| Intended Use | Total RNA preparation for RT-PCR, real-time PCR, library construction and microarray analysis. |
| Principle / Technology | Enhanced chemical and proteolytic lysis, genomic DNA filtration and selective silica-membrane RNA purification. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells and common or difficult animal tissues, including muscle, cartilage, aquatic-animal tissue and fatty tissue. |
| Performance Range / Specifications | Approximately 10-minute workflow with two wash steps; 100 reactions; compatible with routine and complex tissue samples. |
| Specificity | Dedicated genomic DNA filtration and optimized wash chemistry reduce DNA, protein and other tissue-derived contaminants. |
| Reaction Conditions / Protocol | Homogenize and lyse the sample, allow proteolytic digestion where required, remove genomic DNA and insoluble material, bind RNA to the spin column, wash twice and elute. |
| Components / Formulation | Lysis and protease reagents, genomic DNA removal filters, RNA spin columns, wash and elution solutions. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 100 reactions. |
| Product Form | Universal total RNA spin-column kit. |
| Quality Control | RNA yield, purity, integrity and downstream amplification performance are evaluated across common and difficult tissues. |
| Key Features | Compatible with cells, common tissues and difficult high-protein, fibrous or fatty samples; approximately 10-minute workflow; two washes; no phenol/chloroform or β-mercaptoethanol. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | RT-PCR, real-time PCR, library construction and microarray workflows. |
| Application Notes / Precautions | Ensure complete homogenization and use the recommended input range; difficult tissues may require stronger mechanical disruption. |
| Batch-to-Batch Consistency | RNA yield, purity and integrity are controlled across production batches. |
For research use only, not for clinical use.
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