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| Product Name | Ultra-Performance First-Strand cDNA Synthesis Kit with gDNA Removal |
| Catalog No. | NATR-HMM-0176 |
| Description | A high-performance first-strand cDNA synthesis kit with premixed reverse transcriptase, RNase inhibitor and dNTPs, plus a rapid genomic DNA removal module. |
| Intended Use | Reverse transcription; first-strand cDNA synthesis with genomic DNA removal; PCR; qPCR. |
| Principle / Technology | Genomic DNA is removed before a high-performance reverse transcriptase synthesizes first-strand cDNA using a user-selected primer strategy. |
| Sample Type | Total RNA or poly(A)+ RNA. |
| Performance Range / Specifications | 50 or 100 reactions; genomic DNA removal at 42°C for approximately 2 minutes; reverse transcription in approximately 5 minutes; supports cDNA targets up to 15 kb. |
| Specificity | User-selected primers support application-specific cDNA synthesis, including long transcripts and complex RNA templates. |
| Reaction Conditions / Protocol | Remove genomic DNA at 42°C for approximately 2 minutes, then perform reverse transcription for approximately 5 minutes at 37–50°C using the selected RT primer. |
| Components / Formulation | Premixed reverse transcriptase, RNase inhibitor and dNTP mixture; optimized RT buffer; genomic DNA removal module. Reverse-transcription primers are selected according to the application. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions; 100 reactions. |
| Product Form | First-strand cDNA synthesis kit with genomic DNA removal. |
| Quality Control | Evaluated for rapid reverse transcription, long-cDNA synthesis and tolerance to common RNA-sample inhibitors. |
| Key Features | Five-minute reverse transcription; genomic DNA removal; cDNA synthesis up to 15 kb; high inhibitor tolerance; flexible primer selection. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with downstream PCR and qPCR. |
| Application Notes / Precautions | A reaction temperature of 50°C can be used for RNA templates with complex secondary structures. Select oligo(dT), random or gene-specific primers according to the downstream application. |
For research use only, not for clinical use.
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