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| Product Name | Ultra-Fast All-in-One RT SuperMix for qPCR with gDNA Removal |
| Catalog No. | NATR-HMM-0172 |
| Description | An ultra-fast all-in-one reverse-transcription system combining reverse transcriptase, DNase and an optimized buffer to remove genomic DNA and synthesize cDNA in one tube. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; gene expression analysis; two-step RT-qPCR. |
| Principle / Technology | A thermolabile DNase removes genomic DNA while an optimized reverse transcriptase converts RNA to cDNA in the same reaction. |
| Sample Type | RNA from animal cells, animal tissues and plant tissues. |
| Performance Range / Specifications | 100 reactions; genomic DNA removal and reverse transcription completed in approximately 5 minutes. |
| Specificity | Produces qPCR-ready cDNA with high yield, including for low-expression targets. |
| Reaction Conditions / Protocol | Add RNA template and nuclease-free water to the premix; complete genomic DNA removal and reverse transcription in approximately 5 minutes. |
| Components / Formulation | All-in-one mixture containing reverse transcriptase, DNase and an optimized reaction buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions. |
| Product Form | Ultra-fast all-in-one RT supermix. |
| Quality Control | Evaluated with RNA from animal cells, animal tissues and plant tissues followed by qPCR analysis. |
| Key Features | Five-minute workflow; one-tube operation; high cDNA yield; suitable for low-expression targets; simple template-and-water setup. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Resulting cDNA is compatible with dye-based and probe-based qPCR. |
| Application Notes / Precautions | Thaw on ice, mix gently and avoid RNase contamination. |
For research use only, not for clinical use.
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