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| Product Name | Transfection Reagent for Common Cell Lines |
| Catalog No. | RESK-0280 |
| Description | A high-performance cationic polymer transfection reagent designed for plasmid DNA transfection of a wide range of common adherent and suspension cell lines, including HEK293, MCF-7, RAW 264.7, HUVEC, HBE, THP-1, PC-3, A549, HeLa, BJ, U-87 MG, and Caco-2. The reagent features a well-defined structure and uniform molecular weight, combining efficient transfection reagent/DNA complex formation with rapid intracellular DNA release, ensuring excellent transfection performance with minimal cytotoxicity. |
| Intended Use | Plasmid DNA transfection of common adherent or suspension cell lines, including HEK293, MCF-7, RAW 264.7, HUVEC, HBE, THP-1, PC-3, A549, HeLa, BJ, U-87 MG, and Caco-2. For research use only. |
| Principle / Technology | Cationic polymer-based transfection: the polymer forms compact complexes with plasmid DNA, facilitating cellular uptake and rapid intracellular release of the DNA for efficient, highly reproducible transfection. |
| Sample Type | Adherent or suspension cultured mammalian cells |
| Performance Range / Specifications | High transfection efficiency across common cell lines such as HEK293, MCF-7, RAW 264.7, HUVEC, HBE, THP-1, PC-3, A549, HeLa, BJ, U-87 MG, and Caco-2, with highly reproducible results. Low cytotoxicity: cells retain normal morphology and growth after transfection and express high levels of the transfected gene product. Easy to use: no complex pretreatment required; complexes can be added directly to serum-containing medium, reducing handling steps and time. |
| Reaction Conditions / Protocol | Form transfection reagent/DNA complexes and add directly to cells in serum-containing medium; no complex pretreatment is required. |
| Components / Formulation | Cationic polymer-based transfection reagent |
| Storage Conditions | Store at 2-8°C. |
| Shelf Life | 2 years when stored at 2-8°C |
| Package Specifications | 100 uL 1 mL 10 mL |
| Product Form | Liquid |
| Expiration Date / Stability | Stable for 2 years at 2-8°C |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with serum-containing culture medium; suitable for both adherent and suspension cells. |
| Application Notes / Precautions | No complex pretreatment is required; add the complexes directly into serum-containing medium. |
For research use only, not for clinical use.
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