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| Product Name | Suspension Cell Liposomal Transfection Reagent |
| Catalog No. | RESK-0275 |
| Description | This cationic liposomal transfection reagent is specifically optimized for the transfection of suspension cells and is suitable for the delivery of DNA, RNA, and oligonucleotides, providing high transfection efficiency in most eukaryotic cells. The reagent shows low cytotoxicity during transfection; cells maintain good morphology after transfection, grow normally, and express high levels of the transfected gene product. No complex pretreatment is required: the liposome-nucleic acid complex can be added directly to serum-containing medium, reducing handling steps and saving time. It is suitable for both transient and stable transfection experiments. The reagent is supplied as a sterile liquid. |
| Intended Use | For research use in the transfection of DNA, RNA, and oligonucleotides into eukaryotic cells, with particular optimization for suspension cell cultures; suitable for transient and stable transfection. |
| Principle / Technology | Cationic liposomes form complexes with negatively charged nucleic acid molecules through electrostatic interaction. The liposome-nucleic acid complexes are taken up by cells, releasing the nucleic acid cargo for expression. |
| Sample Type | DNA, RNA, and oligonucleotides; suspension and adherent eukaryotic cells |
| Performance Range / Specifications | High transfection efficiency in most eukaryotic cells, especially suspension cells Low cytotoxicity; cells retain normal morphology and growth after transfection Effective for DNA, RNA, and oligonucleotide transfection Compatible with serum-containing medium Suitable for transient and stable transfection |
| Specificity | Specifically optimized for suspension cell transfection; also effective in most eukaryotic cell types |
| Reaction Conditions / Protocol | Add the liposome-nucleic acid complex directly to cells in serum-containing medium. If the medium was not changed before transfection, replace with fresh medium approximately 6 hours after transfection to ensure adequate nutrients. If the medium was changed before transfection, change the medium as usual when nutrients become depleted. |
| Components / Formulation | Cationic liposomal transfection reagent supplied as a sterile liquid |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C |
| Package Specifications | 100 uL 0.5 mL 1.0 mL 5 x 1 mL |
| Product Form | Sterile liquid |
| Shipping Conditions | Shipped on wet ice (ice packs) |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C. Do not freeze; freezing damages the liposomal formulation and reduces transfection performance. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with serum-containing culture medium; suitable for transient and stable transfection of DNA, RNA, and oligonucleotides |
| Application Notes / Precautions | Do not freeze; store at 2-8 °C to maintain optimal transfection performance. For single-plasmid transfection, high efficiency is achieved in validated cell lines. For multi-plasmid co-transfection, the plasmid mixing ratio and the plasmid-to-reagent ratio should be optimized and verified by the user. |
For research use only, not for clinical use.
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