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| Product Name | Streptavidin Magnetic Beads, 5 µm, High Binding Capacity |
| Catalog No. | MAGBEA-0027 |
| Description | Larger 5 µm streptavidin-conjugated superparamagnetic beads with ultra-high biotin binding capacity (>4,000 pmol biotin/mg beads). The increased particle size accommodates larger biotinylated molecules including antibodies, nucleic acids, and protein complexes. Ideal for applications requiring high ligand loading such as chemiluminescence immunoassays and nucleic acid capture for NGS target enrichment. |
| Intended Use | High-capacity biotinylated molecule capture; chemiluminescence immunoassay solid phase; NGS target enrichment and hybrid capture; biotinylated antibody immobilization; aptamer selection (SELEX); DNA-protein interaction studies. |
| Principle / Technology | Recombinant streptavidin tetramer covalently coupled to 5 µm superparamagnetic beads; each streptavidin binds 4 biotin molecules with Kd ~10⁻¹⁵ M, the strongest known non-covalent interaction |
| Detection Method | Wash beads 2× with binding buffer; incubate with biotinylated ligand 30-60 min RT with rotation; magnetic separate; wash 3× to remove unbound ligand; proceed to downstream application |
| Components / Formulation | Streptavidin magnetic beads (10 mg/mL in PBS, 0.1% BSA, 0.02% NaN₃) |
| Storage Conditions | 2-8 °C; do not freeze; mix thoroughly before use |
| Shelf Life | 24 months |
| Package Specifications | 1 mL, 5 mL, 10 mL suspension |
| Product Form | Brown-black magnetic suspension, 10 mg/mL |
| Key Features | Ultra-high capacity >4,000 pmol biotin/mg beads; 5 µm uniform size for large biomolecule capture; recombinant streptavidin — no non-specific binding from avidin glycosylation; extremely stable biotin-streptavidin bond (Kd ~10⁻¹⁵ M); low leaching for repeated use |
| Purity | Recombinant streptavidin >98% SDS-PAGE; endotoxin <0.05 EU/mg; tetramer integrity verified by SEC-HPLC |
| Concentration | 10 mg/mL; biotin binding >4,000 pmol/mg |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Streptavidin tetramer ~53 kDa (monomer ~13.3 kDa) |
| Source / Origin | Recombinant streptavidin expressed in E. coli; ACF |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze |
| Expiration Date / Stability | 24 months at 2-8 °C; never freeze |
| Regulatory / Compliance | For laboratory and research use only; RUO; ACF; ISO 9001 |
| Compatibility | Compatible with biotinylated DNA, RNA, antibodies, peptides, and small molecules; avoid free biotin in buffers; compatible with standard binding/wash buffers; biotin-streptavidin bond stable in 6 M urea, 2% SDS, pH 2-12 |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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