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| Product Name | Magrose NHS (30-150 µm) |
| Catalog No. | SM-HMM-0036 |
| Description | Magrose NHS are porous microspheres based on natural hydrophilic polymer agarose, with particle sizes of 30-150 µm and 10-30 µm. The surface of the NHS-modified beads can form stable peptide bonds with proteins and other molecules with a primary amine moiety, and are used for the affinity purification or analytical detection of antibodies, antigens, and other biomolecules. Compared with traditional carboxyl and amino magnetic beads, magnetic beads with NHS groups on the surface do not need to be activated by EDC/NHS or glutaraldehyde beforehand, and the biologic ligands containing primary amino groups can be covalently coupled to the magnetic beads by simply dissolving the ligands in coupling buffer, and then mixing the protein solution with the NHS beads for 1~2h at room temperature, which is characterized by simple operation, mild coupling conditions, and rapid and efficient coupling of the biological ligands. It has the advantages of simple operation, mild coupling conditions, fast coupling of biological ligands and high efficiency. It is mainly used in the field of separation and purification (such as protein separation and purification, antibody separation and purification). |
| Features | Simple, no need for activation, can be directly covalently coupled with biological ligands; High efficiency, the coupling efficiency of biological ligands can reach more than 90%, which is much higher than that of carboxylated magnetic beads, and the binding load of ligands is higher at the same time; Rapid, 1~2 h to complete the coupling of biological ligands; Mild, room temperature or 4°Ccoupling, the pH of the coupling system is 5~9; Stable, forming stable amide bonds, preventing ligand detachment. Good biocompatibility, reduce non-specific adsorption. |
| Storage | 2-8°C |
| Shelf Life | 1 year |
| Average Particle Size | 30-150 µm |
| Shell | Agarose |
| Preservation Fluid | Anhydrous isopropyl alcohol |
| Concentration | 20% (v/v) |
| Applications | In Vitro Diagnostics
Immunoassays Cell Sorting Immunoprecipitation/co-precipitation Protein/antibody Isolation and Purification |
The development of affinity purification and analytical detection technologies has become a cornerstone of modern biological research, enabling precise isolation and study of biomolecules like antibodies, antigens, and proteins. As research demands grow more rigorous, there is an urgent need for tools that combine efficiency, simplicity, and reliability to streamline experimental workflows.
Magnetic beads have emerged as indispensable materials in this field, thanks to their unique ability to enable rapid separation via magnetic force, reducing processing time and minimizing sample loss. Among various magnetic bead modifications, NHS (N-hydroxysuccinimide) functionalization stands out for its ability to form stable covalent bonds with primary amines—common functional groups in proteins and biomolecular ligands.
Magrose NHS (30-150 µm) is engineered to address the limitations of traditional magnetic beads (such as carboxylated or amino-modified types) that require additional activation steps (e.g., EDC/NHS or glutaraldehyde treatment) before coupling. Built on a natural agarose matrix, this product leverages the hydrophilic and biocompatible properties of agarose to ensure compatibility with biological samples while delivering enhanced coupling performance.
Designed exclusively for research purposes (not for clinical use), Magrose NHS (30-150 µm) caters to the needs of academic laboratories, biotech R&D teams, and research institutions focused on immunoassays, protein purification, cell sorting, and immunoprecipitation applications.
Direct covalent coupling capability without prior activation, eliminating the need for additional reagents like EDC/NHS or glutaraldehyde.
High ligand coupling efficiency of over 90%, outperforming traditional carboxylated magnetic beads in binding performance.
Rapid coupling process that completes within 1–2 hours at room temperature or 4°C, significantly shortening experimental timelines.
Mild reaction conditions with a compatible pH range of 5–9, preserving the structural integrity and biological activity of sensitive biomolecular ligands.
Stable amide bond formation between the beads and ligands, preventing ligand detachment during washing or storage to ensure consistent experimental results.
Natural agarose matrix offers excellent biocompatibility, reducing non-specific adsorption of unwanted biomolecules and improving sample purity.
Porous microsphere structure with an average particle size of 30–150 µm, providing a large surface area for high ligand binding capacity.
Formulated with anhydrous isopropyl alcohol as preservation fluid, ensuring long-term stability when stored at 2–8°C.
Simplifies experimental workflows by removing pre-activation steps, reducing reagent costs, and minimizing the risk of operational errors.
Higher ligand binding load compared to conventional magnetic beads, enabling efficient processing of both small and large sample volumes.
Gentle reaction conditions protect the functionality of biomolecules, making it suitable for fragile ligands like antibodies and recombinant proteins.
Consistent performance across batches, ensuring reproducibility in research experiments and reducing experimental variability.
Versatile compatibility with multiple applications, including in vitro diagnostics research, immunoassays, cell sorting, and immunoprecipitation/co-precipitation.
Easy to use with straightforward protocols—simply dissolve ligands in coupling buffer, mix with the beads, and incubate to achieve covalent coupling.
Biocompatible agarose base minimizes sample contamination and non-specific interactions, enhancing the accuracy of downstream analyses.
1-year shelf life under recommended storage conditions, providing long-term usability for research projects.
For research use only, not for clinical use.
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