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| Product Name | Magrose Protein A (30-150 µm) |
| Catalog No. | SM-HMM-0054 |
| Description | Protein A/G Antibody Purification Magnetic Beads series products utilize nano-surface technology to encapsulate Protein A/G on the surface of superparamagnetic microspheres in a high density orientation. The product has higher antibody binding capacity and lower protein non-specific adsorption rate, more uniform elution conditions, one-step purification can be separated from serum samples with purity greater than 90% of the antibody. The product is nano-sized magnetic microspheres with large specific surface area, which can greatly shorten the time required for antibody adsorption, and skilled operation can complete the antibody adsorption process within 10min, and complete the antibody purification process within 30min. The antibody purification magnetic bead kit is equipped with optimized pre-made buffer, which provides the best reaction conditions for antibody purification experiments and improves the stability of antibody purification experiments. |
| Features | Highly efficient antibody binding capacity and ultra-low non-specific adsorption; Time-saving, easy and gentle operatio; High product stability; Antibody elution system closer to neutrality; Extremely low Protein A ligand shedding. Reusable. |
| Storage | 2-8°C |
| Shelf Life | 2 years |
| Average Particle Size | 30-150 µm |
| Preservation Fluid | PBST, 0.1% (V/V) proclin-300 |
| Concentration | 10% (v/v) |
Antibody purification is a critical step in life science research, enabling the isolation of high-purity antibodies for applications such as Western blotting, ELISA, immunoprecipitation, and flow cytometry. The quality of purified antibodies directly impacts the accuracy, reproducibility, and reliability of experimental results, making the selection of efficient purification tools essential for researchers worldwide.
Traditional antibody purification methods, such as column chromatography, often involve complex workflows, long processing times, and high non-specific adsorption, leading to reduced antibody yield and purity. To address these challenges, magnetic bead-based purification technologies have emerged as a revolutionary solution, leveraging the unique properties of superparamagnetic microspheres to simplify workflows and enhance performance.
Magrose Protein A (30-150 µm) is developed based on advanced nano-surface modification technology, combining the high affinity of Protein A for antibodies with the convenience of magnetic separation. Protein A, a bacterial protein derived from Staphylococcus aureus, specifically binds to the Fc region of IgG antibodies from various species (including human, rabbit, mouse, and goat), enabling selective capture and purification of target antibodies from complex samples like serum, cell culture supernatants, and hybridoma supernatants.
High-efficiency antibody binding: Utilizes high-density oriented immobilization of Protein A on superparamagnetic microspheres, ensuring strong and specific binding to IgG antibodies, resulting in exceptional binding capacity.
Ultra-low non-specific adsorption: The nano-engineered surface of the magnetic beads minimizes non-specific binding to contaminating proteins, lipids, and other impurities, ensuring high-purity antibody elution.
Time-saving workflow: The large specific surface area of the 30-150 µm microspheres enables rapid antibody adsorption (completable within 10 minutes with proficient operation) and full purification within 30 minutes, significantly reducing experimental time compared to traditional methods.
Gentle and user-friendly operation: Magnetic separation eliminates the need for centrifugation or column packing, reducing mechanical stress on antibodies and simplifying the workflow—no specialized equipment or technical expertise is required.
High product stability: Maintains consistent performance over a 2-year shelf life when stored at 2-8°C, with stable binding affinity and minimal degradation, ensuring reliable experimental results batch after batch.
Near-neutral elution conditions: The optimized elution system operates under conditions closer to neutral pH, reducing damage to antibody structure and biological activity, preserving antibody functionality for downstream applications.
Extremely low ligand shedding: Advanced surface modification technology ensures firm immobilization of Protein A, minimizing ligand shedding during purification and avoiding contamination of the final antibody product.
Reusable design: The magnetic beads can be regenerated and reused multiple times with proper handling, reducing experimental costs without compromising performance.
Ready-to-use buffers included: The kit comes with pre-optimized PBST-based preservation fluid (containing 0.1% (V/V) proclin-300) and matching buffers, providing optimal reaction conditions and enhancing the reproducibility of purification experiments.
Superior purity guarantee: Enables one-step purification of antibodies with purity exceeding 90% from serum samples, meeting the high standards required for sensitive research applications.
Broad sample compatibility: Effectively purifies IgG antibodies from a wide range of sample types, including serum, plasma, cell culture supernatants, and hybridoma supernatants, adapting to diverse research needs.
Cost-effective: Reusable magnetic beads and pre-made buffers reduce the need for additional reagents and consumables, lowering overall experimental costs while maintaining high performance.
Scalable performance: Available in multiple volume options (50 mL, 100 mL, 500 mL), suitable for both small-scale experiments (e.g., academic research projects) and large-scale antibody production (e.g., biotechnology R&D).
Preserves antibody activity: Gentle adsorption and near-neutral elution minimize denaturation or inactivation of antibodies, ensuring the purified product retains its binding specificity and biological function for downstream assays.
Consistent and reliable results: Strict quality control during production ensures batch-to-batch consistency in particle size, concentration (10% v/v), and binding capacity, eliminating variability in experimental outcomes.
Research-focused design: Tailored exclusively for research use, with specifications and performance optimized to address the unique needs of life science researchers, from basic research to preclinical development.
For research use only, not for clinical use.
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