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| Product Name | Anti-Mouse IgG Magnetic Beads, 2 µm, for Immunoprecipitation |
| Catalog No. | MAGBEA-0024 |
| Description | Superparamagnetic beads (2 µm) covalently conjugated with polyclonal goat anti-mouse IgG (H+L) antibodies. Designed for rapid, high-capacity immunoprecipitation of mouse primary antibodies and their target antigen complexes. The uniform 2 µm particle size provides high surface area for maximum binding capacity while ensuring rapid magnetic separation in manual and automated workflows. |
| Intended Use | Immunoprecipitation with mouse primary antibodies; target protein enrichment; protein complex purification; chromatin immunoprecipitation (ChIP); antibody depletion; automated IP platforms. |
| Principle / Technology | Goat anti-mouse IgG antibodies covalently coupled to superparamagnetic iron oxide core with hydrophilic polymer coating; Fc-region recognition captures mouse IgG subclasses (IgG1, IgG2a, IgG2b, IgG3) |
| Detection Method | Add 25-50 µL bead slurry per IP; wash 2× with binding buffer; incubate with antibody-lysate mixture 30-60 min at RT or 4 °C overnight; magnetic separate; wash 3-5×; elute |
| Components / Formulation | Anti-mouse IgG magnetic beads (10 mg/mL suspension in PBS with 0.1% BSA, 0.02% NaN₃) |
| Storage Conditions | 2-8 °C; do not freeze; keep suspension homogeneous — mix before each use |
| Shelf Life | 24 months from manufacture date |
| Package Specifications | 1 mL, 5 mL, 10 mL suspension |
| Product Form | Brown-black magnetic suspension; 10 mg beads/mL |
| Key Features | Binding capacity >10 µg mouse IgG per mg beads; 2 µm uniform particles; rapid <10 sec magnetic separation; low non-specific binding; goat antibody — no protein A/G cross-reactivity; compatible with manual and automated platforms |
| Purity | Goat anti-mouse IgG affinity-purified; endotoxin <0.1 EU/mg; no cross-reactivity with rat, rabbit, or human IgG |
| Concentration | 10 mg beads/mL suspension; approximately 5×10⁸ beads/mg |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Goat polyclonal anti-mouse IgG (H+L); recombinant protein-free beads |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze; ship within 72 hr |
| Expiration Date / Stability | 24 months at 2-8 °C; never freeze — irreversible aggregation; resuspend thoroughly before each use; do not allow beads to dry |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with mouse IgG1, IgG2a, IgG2b, IgG3; not compatible with mouse IgM or IgA; avoid SDS >0.1%, urea >2 M, guanidine >1 M; compatible with common IP lysis buffers (RIPA, NP-40, Triton X-100) |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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