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| Product Name | Stem-Loop miRNA First-Strand cDNA Synthesis Kit with gDNA Removal |
| Catalog No. | NATR-HMM-0181 |
| Description | A stem-loop reverse-transcription kit for sensitive miRNA cDNA synthesis, incorporating a rapid genomic DNA removal step before reverse transcription. |
| Intended Use | First-strand cDNA synthesis for stem-loop miRNA assays and subsequent miRNA qPCR. |
| Principle / Technology | Genomic DNA is removed before a thermostable reverse transcriptase and miRNA-specific stem-loop primer system convert target miRNA into cDNA. |
| Sample Type | Total RNA or miRNA preparations. |
| Performance Range / Specifications | 50 or 100 reactions at 20 µL per reaction; a broad tested RNA input range from 10 pg to 1 µg. |
| Sensitivity / LOD | Supports detection from picogram-level RNA input. |
| Specificity | Dedicated stem-loop reverse transcription improves target-oriented miRNA cDNA synthesis. |
| Reaction Conditions / Protocol | Remove genomic DNA at 42°C for approximately 2 minutes, then perform stem-loop reverse transcription using the recommended program. |
| Components / Formulation | Thermostable reverse-transcription system, genomic DNA removal reagents and stem-loop miRNA reverse-transcription reagents. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 50 reactions (20 µL per reaction); 100 reactions (20 µL per reaction). |
| Product Form | Stem-loop miRNA first-strand cDNA synthesis kit. |
| Key Features | Rapid genomic DNA removal; picogram-level sensitivity; broad RNA input range; optimized stem-loop miRNA reverse transcription. |
| Compatibility | Resulting cDNA is suitable for downstream stem-loop miRNA qPCR. |
For research use only, not for clinical use.
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