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| Product Name | Stable Pre-Mixed Multiplex Probe qPCR Premix |
| Catalog No. | NATR-HMM-0195 |
| Description | A concentrated multiplex probe qPCR premix developed for high specificity and stable pre-mixing with assay primers and probes. |
| Intended Use | Multiplex quantification; gene-expression analysis; pathogen detection; tumor-related nucleic acid testing. |
| Principle / Technology | A hot-start polymerase controlled by dual-species antibodies, an optimized multiplex buffer and a dUTP/UDG system support specific probe-based amplification while enabling stable primer/probe pre-mixing. |
| Detection Method | Multiplex fluorescent probe-based real-time PCR. |
| Sample Type | DNA templates. |
| Performance Range / Specifications | 200, 500 or 5,000 reactions; supports at least 10-plex amplification under evaluated conditions and templates spanning approximately 20–75% GC content. |
| Specificity | High-specificity hot-start control is designed to reduce nonspecific multiplex amplification. |
| Reaction Conditions / Protocol | Prepare the reaction with the 5× premix, primers, probes and DNA template, then run a compatible multiplex probe qPCR program. |
| Components / Formulation | 5× multiplex probe qPCR premix containing hot-start DNA polymerase, optimized multiplex buffer and dUTP/UDG carryover-control system. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 500 reactions; 5,000 reactions. |
| Product Form | 5× high-specificity multiplex probe qPCR premix. |
| Quality Control | Multiplex specificity, plex level, GC compatibility and accelerated primer/probe premix stability are evaluated by real-time PCR. |
| Key Features | High specificity; supports high-plex assays; stable primer/probe pre-mixing; broad GC compatibility; carryover contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Primer/probe premixes prepared with the system remained stable for 28 days at 37°C and through 50 freeze–thaw cycles under the evaluated accelerated conditions. |
| Compatibility | Suitable for scalable multiplex DNA-target assays on common probe-based real-time PCR platforms. |
For research use only, not for clinical use.
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