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Stable Pre-Mixed Multiplex Probe qPCR Premix

Cat.No: NATR-HMM-0195 Datasheet

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Product Name Stable Pre-Mixed Multiplex Probe qPCR Premix
Catalog No. NATR-HMM-0195
Description A concentrated multiplex probe qPCR premix developed for high specificity and stable pre-mixing with assay primers and probes.
Intended Use Multiplex quantification; gene-expression analysis; pathogen detection; tumor-related nucleic acid testing.
Principle / Technology A hot-start polymerase controlled by dual-species antibodies, an optimized multiplex buffer and a dUTP/UDG system support specific probe-based amplification while enabling stable primer/probe pre-mixing.
Detection Method Multiplex fluorescent probe-based real-time PCR.
Sample Type DNA templates.
Performance Range / Specifications 200, 500 or 5,000 reactions; supports at least 10-plex amplification under evaluated conditions and templates spanning approximately 20–75% GC content.
Specificity High-specificity hot-start control is designed to reduce nonspecific multiplex amplification.
Reaction Conditions / Protocol Prepare the reaction with the 5× premix, primers, probes and DNA template, then run a compatible multiplex probe qPCR program.
Components / Formulation 5× multiplex probe qPCR premix containing hot-start DNA polymerase, optimized multiplex buffer and dUTP/UDG carryover-control system.
Storage Conditions Store at −30 to −15°C.
Package Specifications 200 reactions; 500 reactions; 5,000 reactions.
Product Form 5× high-specificity multiplex probe qPCR premix.
Quality Control Multiplex specificity, plex level, GC compatibility and accelerated primer/probe premix stability are evaluated by real-time PCR.
Key Features High specificity; supports high-plex assays; stable primer/probe pre-mixing; broad GC compatibility; carryover contamination control.
Shipping Conditions Ship at or below 0°C.
Expiration Date / Stability Primer/probe premixes prepared with the system remained stable for 28 days at 37°C and through 50 freeze–thaw cycles under the evaluated accelerated conditions.
Compatibility Suitable for scalable multiplex DNA-target assays on common probe-based real-time PCR platforms.

For research use only, not for clinical use.

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