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| Product Name | Small Molecule Compound Transfection Reagent, 1 mg/mL |
| Catalog No. | RERE-0323 |
| Description | An economical and dependable reagent for transient delivery work. In HEK293 and CHO expression systems it sustains consistently high gene expression across production vessels spanning 96-well plates up to 100 L bioreactors. The sterile solution is supplied ready to use at 1 mg/mL. The working ratio of cargo to reagent is 1:3, so 1 mL of the product is sufficient for delivering 330 µg of cargo. Expression is typically detectable 36 to 48 hours after delivery, with peak levels generally observed at 72 to 96 hours. |
| Intended Use | Transient delivery in HEK293 and CHO expression systems across scales from 96-well plates to 100 L bioreactors. |
| Principle / Technology | The reagent complexes the cargo at a defined 1:3 ratio and escorts it into cells, where expression becomes measurable within two days and peaks around three to four days. |
| Specificity | Sterile solution; 1.0 mg/mL; BioReagent grade; for cell culture; molecular biology grade. |
| Reaction Conditions / Protocol | Plate cells 18-24 hours in advance to reach 60-80% confluence. Dilute 2 µg of cargo in 300 µL of serum-free medium, add 6 µL of reagent, mix and stand for 30 minutes at room temperature under sterile conditions, then transfer the complex to the wells. Replace with fresh medium 12-18 hours after delivery. Avoid antibiotics during the process and use endotoxin-free nucleic acid preparations with a 260/280 ratio of 1.8-2.0. |
| Storage Conditions | -20°C; avoid repeated freeze-thaw cycles; aliquot on first use; 12 months. |
| Package Specifications | 100 µL, 500 µL, 1 mL |
| Key Features | Consistent high expression in HEK293 and CHO platforms; single 1 mL vial delivers up to 330 µg of cargo; scalable from screening plates to 100 L bioreactors. |
For research use only, not for clinical use.
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