- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | siRNA Transfection Reagent for Primary and Hard-to-Transfect Cells |
| Catalog No. | RESK-0585 |
| Description | This siRNA Transfection Reagent is a lipid nanoparticle (LNP)-based in vitro transfection reagent built on a proprietary delivery platform. It efficiently delivers siRNA into cells and achieves >90% target gene silencing even in difficult systems such as primary cells. Complex preparation is completed in 5 min, making it dedicated to gene knockdown in primary cells and other hard-to-transfect cell lines. Transfection case data are available for more than 100 cell types. |
| Intended Use | For efficient delivery of siRNA into primary cells and hard-to-transfect cell lines to achieve target gene knockdown in RNAi experiments. For research use only. |
| Principle / Technology | Lipid nanoparticle (LNP)-based delivery. The reagent forms lipid nanoparticle complexes with siRNA, which are taken up by cells and release the siRNA intracellularly to mediate RNA interference and silence the target gene. |
| Sample Type | Primary cells and hard-to-transfect cell lines; siRNA. |
| Performance Range / Specifications | Higher transfection efficiency: >90% target gene knockdown even in hard-to-transfect primary cells Lower siRNA requirement: high knockdown efficiency is maintained across an siRNA working range of 2-50 nM Minimalist operation: complex preparation is completed in 5 min; compatible with serum and antibiotics, with no medium change required |
| Reaction Conditions / Protocol | Mix the reagent with siRNA (2-50 nM final concentration) to form complexes in approximately 5 min, then add the complexes directly to the cells. The reagent is compatible with serum- and antibiotic-containing medium; no medium change is required after transfection. |
| Components / Formulation | Lipid nanoparticle (LNP)-based siRNA transfection reagent, supplied as a ready-to-use liquid reagent. |
| Storage Conditions | Store at 2-8 °C. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 25 μL; 100 μL; 0.75 mL; 1.5 mL |
| Product Form | Liquid reagent. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with serum and antibiotics (no medium change required). Validated for transfection in more than 100 cell types, including primary cells and hard-to-transfect cell lines. |
| Application Notes / Precautions | In head-to-head testing against comparable market products, this reagent delivered significantly stronger target gene knockdown across multiple cell lines. Use siRNA within the recommended 2-50 nM range for optimal performance. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
