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| Product Name | Single-Stranded DNA Methylation Library Prep Kit for mainstream sequencing Platforms |
| Catalog No. | REKI-0049 |
| Description | A single-stranded DNA methylation library prep kit designed for mainstream sequencing sequencing platforms, with improved adapter ligation and a newer high-fidelity polymerase for uniform, faithful amplification. The single-stranded chemistry, running from denaturation through 3-prime and 5-prime adapter ligation with strand extension between, offers a more economical and faster route than conventional double-stranded methylation library methods. |
| Intended Use | Methylation library construction from regular, trace or bisulfite-converted samples for mainstream sequencing platform sequencing. |
| Principle / Technology | DNA denaturation, 3-prime adapter ligation, complementary strand extension, 5-prime adapter ligation and amplification convert fragments into mainstream-sequencing compatible methylation libraries. |
| Sample Type | Genomic DNA, FFPE DNA, cfDNA, ChIP DNA |
| Performance Range / Specifications | Input 10 pg to 250 ng; accepts fragments down to 40 bp; library prep approximately 2 hours |
| Specificity | New high-fidelity polymerase; improved ligation efficiency |
| Storage Conditions | BOX I: -25 to -15°C, 1 year; BOX II: -25 to -15°C, 18 months |
| Package Specifications | 8 T / 24 T |
| Product Form | Two-box kit |
| Key Features | Economical single-stranded chemistry; works on degraded and trace inputs; about 2 hours to complete |
| Compatibility | Compatible with mainstream sequencing high-throughput sequencing platforms |
For research use only, not for clinical use.
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