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| Product Name | Single-Phase Chloroform-Free Total RNA Isolation Reagent |
| Catalog No. | DREK-0104 |
| Description | A single-phase RNA isolation reagent that avoids chloroform and multiphase separation. The chemistry supports total RNA and small RNA recovery from animal tissues, plant tissues, cultured cells and microorganisms. |
| Intended Use | Total RNA and small RNA isolation for cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting and high-throughput sequencing. |
| Principle / Technology | Chemical lysis and single-phase impurity removal followed by alcohol precipitation and washing, without chloroform partitioning. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Animal tissues, plant tissues, cultured cells and microorganisms. |
| Performance Range / Specifications | Room-temperature single-phase workflow; no chloroform or β-mercaptoethanol; supplied in 100 mL and 200 mL formats. |
| Specificity | The single-phase chemistry isolates total RNA and small RNA while reducing protein, DNA and other sample contaminants. |
| Reaction Conditions / Protocol | Homogenize the sample in the reagent, perform the specified clarification step without phase separation, precipitate RNA, wash and dissolve. |
| Components / Formulation | Ready-to-use single-phase total RNA isolation reagent. |
| Package Specifications | 100 mL; 200 mL. |
| Product Form | Liquid chloroform-free RNA isolation reagent. |
| Quality Control | RNA yield, purity and integrity are evaluated with representative animal, plant and cultured-cell samples. |
| Key Features | No chloroform; no multiphase extraction; room-temperature operation; total RNA and small RNA recovery; broad sample compatibility. |
| Compatibility | cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting and sequencing workflows. |
| Application Notes / Precautions | Use RNase-free consumables and thoroughly homogenize difficult samples before clarification. |
| Batch-to-Batch Consistency | RNA yield, purity and integrity are controlled across production batches. |
For research use only, not for clinical use.
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