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| Product Name | Single-Fragment One-Step Seamless Cloning Kit |
| Catalog No. | NATR-HMM-0163 |
| Description | A ligase-independent one-step cloning system for directional insertion of a single DNA fragment into virtually any linearized vector. |
| Intended Use | Rapid cloning, high-throughput cloning, seamless cloning, site-directed mutagenesis and double-stranded DNA assembly. |
| Principle / Technology | Enhanced recombinase and optimized reaction buffer assemble vector and insert through terminal homology while minimizing vector self-ligation. |
| Sample Type | Linearized cloning vectors and single PCR-derived DNA inserts. |
| Performance Range / Specifications | Supports DNA inserts from 50 bp to 10 kb and plasmid-independent vector choices. |
| Specificity | Reported positive clone rate exceeds 95%. |
| Reaction Conditions / Protocol | Incubate the recombination reaction for 30 minutes before transformation. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 25 reactions; 50 reactions. |
| Product Form | Single-fragment seamless cloning kit. |
| Key Features | Ligase-independent workflow; low vector background; no restriction-site limitation within the insert; accepts unpurified linearized vector and PCR products; broad 50 bp to 10 kb insert range. |
| Compatibility | Compatible with linearized vectors and unpurified PCR products. |
For research use only, not for clinical use.
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