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| Product Name | Single-Cell Sequence-Specific Preamplification Kit |
| Catalog No. | NATR-HMM-0186 |
| Description | A one-tube sequence-specific preamplification system for targeted transcript analysis from single cells or other very low-input RNA samples. |
| Intended Use | Single-cell gene-expression analysis; low-input targeted transcript preamplification; qPCR target enrichment. |
| Principle / Technology | Cell lysis, reverse transcription and sequence-specific PCR preamplification are combined in one tube to enrich selected transcripts before real-time qPCR. |
| Detection Method | Downstream real-time qPCR. |
| Sample Type | Single cells or low-input cell populations. |
| Performance Range / Specifications | 200 reactions; supports approximately 1–1,000 cells and simultaneous preamplification of up to 500 target genes. |
| Sensitivity / LOD | Suitable for single-cell and other low-input samples. |
| Specificity | Target enrichment is driven by the user-defined sequence-specific primer pool. |
| Reaction Conditions / Protocol | Combine the sample with the selected primer pool and supplied reagents for one-tube lysis, reverse transcription and preamplification, then dilute or transfer the product to real-time qPCR. |
| Components / Formulation | One-tube cell-lysis, reverse-transcription and sequence-specific preamplification reagents. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions. |
| Product Form | Single-cell targeted preamplification kit. |
| Key Features | One-tube workflow; single-cell sensitivity; up to 500 target genes; reduced handling and contamination risk; broad qPCR-instrument compatibility. |
| Shipping Conditions | Ship at −20 to 0°C. |
| Compatibility | Preamplified products are compatible with real-time qPCR instruments. |
| Application Notes / Precautions | For a 5 µL reaction, keep the sample volume at or below approximately 0.5 µL when possible. |
For research use only, not for clinical use.
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