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| Product Name | Silica-Coated Magnetic Beads for RNA Extraction |
| Catalog No. | MAGBEA-0004 |
| Description | Silica-coated superparamagnetic beads specifically surface-optimized for total RNA capture and purification. The proprietary surface modification incorporates mixed silanol and diol functional groups that exhibit enhanced hydrogen bonding with the 2'-hydroxyl group of ribose, providing preferential RNA binding characteristics under controlled binding conditions. The RNase-free manufacturing process, validated through rigorous enzyme activity testing, ensures that isolated RNA maintains integrity for sensitive downstream applications including RNA sequencing and quantitative PCR. |
| Intended Use | Capture and purification of total RNA from cell lysates, tissue homogenates, and enzymatic reactions for gene expression analysis, transcriptomics, and custom RNA extraction system development. |
| Principle / Technology | Superparamagnetic iron oxide cores are coated with a silanol/diol-functionalized silica surface that provides enhanced affinity for RNA through dual hydrogen-bonding interactions with the ribose sugar backbone. Under optimized binding conditions with guanidine isothiocyanate and ethanol, RNA binds selectively while DNA and proteins are removed by washing. The bound RNA is eluted under mild conditions that preserve RNA integrity. All manufacturing steps are conducted under RNase-free conditions with DEPC-treated water and certified RNase-free reagents. |
| Detection Method | DLS for particle characterization; UV spectrophotometry for RNA quantification and purity; Bioanalyzer/TapeStation for RNA integrity assessment; RT-qPCR of reference genes for functional integrity; fluorometric quantitation with RNA-specific dyes; RNase activity testing with fluorescent RNase substrate |
| Sample Type | Total RNA from cultured cells, animal tissues, bacterial cells, yeast, and plant tissues; compatible with TRIzol/TRI Reagent-based extraction protocols and guanidine-based lysis buffer systems; cell lysates prepared with guanidine isothiocyanate-containing lysis buffers; RNA in aqueous solution after organic extraction |
| Performance Range / Specifications | Particle diameter: 200 ± 30 nm (DLS); PDI: <0.20; saturation magnetization: 40-55 emu/g; RNA binding capacity: 30-60 µg total RNA per mg beads; RNA recovery: >85% for total RNA including mRNA, rRNA, and miRNA; A260/A280 of eluted RNA: 1.9-2.1; RNase: not detected; DNase: not detected |
| Sensitivity / LOD | Effective RNA recovery from as few as 100 cells; detection of low-abundance mRNA (<5 copies/cell) from eluted RNA; miRNA recovery comparable to dedicated miRNA isolation products; quantitative RNA binding from solutions containing ≥5 ng/mL RNA |
| Specificity | Total RNA recovery without size bias; mRNA, rRNA, tRNA, and miRNA recovered in proportions similar to input; minimal DNA carryover (<1% of total nucleic acid); protein contamination <0.2%; no detectable genomic DNA in eluate by qPCR with intron-spanning primers; RNase-free certification for all manufactured lots |
| Reaction Conditions / Protocol | Vortex bead vial for 60 seconds; add bead slurry (10-50 µL) to RNA solution containing 1-2 volumes binding buffer (guanidine isothiocyanate, ethanol); incubate at room temperature for 5-10 minutes; place on magnetic rack for 1-2 minutes; aspirate supernatant; wash beads with 70% ethanol (prepared with DEPC-treated water) twice; air-dry beads for 2-5 minutes (do not over-dry); elute RNA in 20-50 µL RNase-free water or TE; incubate at 56°C for 5 minutes; separate beads; transfer RNA supernatant to clean RNase-free tube; protocol time: 20-30 minutes |
| Components / Formulation | Silica-coated superparamagnetic beads with diol/silanol surface functionalization, suspended at 20-50 mg/mL in DEPC-treated PBS pH 7.4 with 0.02% sodium azide; all components manufactured and packaged under RNase-free conditions |
| Storage Conditions | 2-8°C in sealed RNase-free container; protect from light; do not freeze; use aseptic technique when handling; dedicated RNase-free workspace recommended; do not share pipettes or reagents with RNase-contaminated areas |
| Shelf Life | 24 months at 2-8°C when handled with RNase-free technique; RNase-free integrity maintained throughout stated shelf life; repeated container opening increases contamination risk—consider single-use aliquots for critical work |
| Package Specifications | 1 mL, 5 mL, 10 mL suspensions at 20 mg/mL and 50 mg/mL; supplied in RNase-free vials; certified free of RNase activity by lot-specific testing |
| Product Form | Dark brown to black RNase-free aqueous suspension; homogeneous after vortexing; no visible aggregates or sediment after resuspension; free of detectable RNase activity (fluorescent substrate assay) |
| Quality Control | Stringent QC including: RNase activity test (≤0.01 Kunitz units per mg beads), DNase activity test (negative by pUC19 incubation assay), RNA binding capacity with HeLa total RNA standard, particle size, magnetic separation speed, endotoxin (<0.05 EU/mg), sterility; RNase-free certification per lot |
| Key Features | Optimized diol/silanol surface for enhanced RNA binding; RNase-free manufacturing throughout; broad RNA size range recovery including miRNA; compatible with standard and automated magnetic separation; DEPC-treated water used throughout production; validated for downstream RNA-seq and RT-qPCR; low elution volume for concentrated RNA recovery |
| Purity | >98% magnetite/silica; RNase-free certified; DNase-free certified; endotoxin <0.05 EU/mg; trace metals below detection; DEPC-treated process water |
| Concentration | 20-50 mg/mL as specified on lot label; gravimetric solids analysis per batch; particle count approximately 10^11-10^12 particles per mL |
| Activity / Unit Definition | RNA binding capacity: 30-60 µg/mg (HeLa total RNA standard in guanidine isothiocyanate/ethanol binding buffer); binding capacity includes mRNA, rRNA, and small RNA fractions |
| Molecular Weight | Not applicable for nanoparticle materials; amorphous silica shell, magnetite core; no discrete molecular weight |
| Source / Origin | Wholly synthetic; magnetite cores by co-precipitation; silica/diol coating by sequential sol-gel process using TEOS and glycidoxypropyltrimethoxysilane modifiers; all reagents are synthetic, DEPC-treated; no biological source materials; manufactured in RNase-free ISO Class 7 cleanroom |
| pH Range / Optimal pH | Stable suspension pH 4.0-10.0; optimal RNA binding pH 5.0-6.5; optimal RNA elution pH 7.0-8.0; irreversible aggregation below pH 3.5; silica dissolution above pH 11 |
| Shipping Conditions | Ambient temperature with cold packs for summer transit; protect from freezing; RNase-free packaging integrity maintained during shipping; non-hazardous classification; standard courier acceptable |
| Expiration Date / Stability | 24 months at 2-8°C; RNase-free status maintained through expiry when handled aseptically; accelerated testing at 37°C/75% RH for 21 days: RNA binding capacity >85% of initial, no detectable RNase; preservative efficacy verified for full shelf life |
| Regulatory / Compliance | For research use and OEM product development only; not for diagnostic applications without appropriate regulatory clearance; manufactured under ISO 9001; RNase-free process certification; DEPC treatment compliance with recommended safety guidelines |
| Compatibility | Compatible with guanidine isothiocyanate and guanidine hydrochloride-based lysis/binding buffers; compatible with TRIzol/TRI Reagent-based phase separation followed by bead capture; works with standard magnetic racks and automated platforms (KingFisher, Maxwell); eluted RNA suitable for RT-qPCR, RNA-seq, microarray |
| Recommended Buffer System | Storage: DEPC-treated PBS pH 7.4 with sodium azide; binding: guanidine isothiocyanate buffer with ethanol (user-supplied or custom); wash: 70-80% ethanol in DEPC-treated water; elution: DEPC-treated water or RNase-free TE buffer |
| Application Notes / Precautions | All work should be performed in an RNase-free environment with dedicated RNase-free equipment; pre-aliquot bead suspension into single-use volumes to minimize contamination risk; if performing organic extraction (TRIzol method), ensure complete removal of phenol before bead binding; use RNase-free pipette tips exclusively; do not touch bead vial rim with non-sterile gloves; for low-yield samples, reduce elution volume and extend incubation time to 10 minutes |
| Batch-to-Batch Consistency | RNA binding capacity CV <12% between lots; RNase-free verification per lot; particle size CV <15%; magnetic separation speed consistency <10%; Diol surface functionalization density controlled by TOF-SIMS per batch; comprehensive lot certification |
For research use only, not for clinical use.
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