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| Product Name | Silica-Coated Magnetic Beads for DNA Extraction (500 nm) |
| Catalog No. | MAGBEA-0003 |
| Description | Large-diameter 500 nm silica-coated superparamagnetic beads engineered for ultra-fast magnetic separation in high-throughput and automated nucleic acid extraction workflows. The increased particle size provides significantly enhanced magnetic responsiveness, enabling near-instantaneous bead capture (<5 seconds) even with simple magnetic racks. The silica coating incorporates optimized pore architecture that maintains adequate DNA binding capacity despite the reduced surface-area-to-volume ratio of larger particles, making these beads ideal for applications where processing speed takes priority. |
| Intended Use | Rapid DNA purification in automated high-throughput extraction systems, field-deployable nucleic acid testing devices, and point-of-care diagnostic platform development; especially suited for applications requiring fast magnetic separation with minimal dwell time. |
| Principle / Technology | Large superparamagnetic iron oxide cores (approximately 400-450 nm) are coated with a 25-50 nm thick silica layer through a multi-step sol-gel process. The silica surface chemistry facilitates DNA binding via chaotropic salt-mediated interaction with surface silanol groups. The large magnetic core diameter results in high magnetic moment per particle, enabling magnetic separation within seconds using standard permanent magnets. DNA release is achieved through pH and ionic strength modulation. |
| Detection Method | DLS and laser diffraction for hydrodynamic size; SEM and TEM for morphological analysis; SQUID or VSM magnetometry for magnetic properties; DNA binding and recovery quantification by UV spectrophotometry and qPCR; automated liquid handler integration testing for separation speed validation |
| Sample Type | DNA in solution including genomic DNA, PCR amplicons, plasmid DNA, and NGS library fragments; optimized for binding buffers containing guanidine salts at concentrations of 1-6 M with 30-70% alcohol (ethanol or isopropanol); suitable for cell lysates and tissue homogenates after protein removal |
| Performance Range / Specifications | Particle diameter: 500 ± 60 nm (DLS); PDI: <0.15; saturation magnetization: 50-70 emu/g; magnetic separation time: <5 seconds (standard magnetic rack, 1.5 mL tube); DNA binding capacity: 20-40 µg/mg (500 bp fragment); surface area: 5-10 m²/g; sedimentation rate: faster than 50-200 nm particles—vortex immediately before use |
| Sensitivity / LOD | Effective DNA recovery from solutions containing ≥20 ng/mL DNA; PCR product purification efficiency >90% for 100 bp-2 kb fragments; consistent performance in 96-well plate format with <8% well-to-well variation |
| Specificity | Selective for DNA over RNA and protein under optimized binding conditions; RNA carryover <8% under standard conditions; protein contamination <0.5% with adequate wash steps; no interference with PCR or sequencing when washing protocol is followed; DNase/RNase free |
| Reaction Conditions / Protocol | Vigorously vortex bead suspension for 60 seconds; add 5-50 µL bead slurry to DNA-containing solution with binding buffer; incubate 3-5 minutes with agitation (shorter incubation acceptable due to large surface binding kinetics); place on magnetic rack—separation complete within 5 seconds; wash beads with 70-80% ethanol 1-2 times; air-dry for 2-3 minutes; elute in 20-100 µL TE buffer at 56°C for 5 minutes; total protocol: 15-20 minutes; well-suited for 96-well magnetic plate processing |
| Components / Formulation | Silica-coated superparamagnetic iron oxide microparticles, 20-50 mg/mL aqueous suspension (deionized water with 0.02-0.05% ProClin or sodium azide preservative); bead count: approximately 10^9 particles per mg; each bead contains approximately 10^7-10^8 magnetic nanocrystals |
| Storage Conditions | 2-8°C in sealed container; protect from light; do not freeze; store upright; vortex thoroughly before each use; avoid prolonged exposure to air when container is open |
| Shelf Life | 24 months at 2-8°C; real-time stability demonstrated for 24 months; accelerated aging studies at 45°C for 21 days show <15% performance degradation; colloidal stability maintained throughout shelf life |
| Package Specifications | 1 mL, 5 mL, 10 mL, 50 mL, 100 mL, and bulk packaging; concentrations: 10, 20, 50 mg/mL; OEM and custom packaging available; supplied in glass or PET bottles |
| Product Form | Dark brown to nearly black suspension; rapid settling upon standing (vortex to resuspend); homogeneous caramel-to-black color when resuspended; no clumps or granular texture after vortexing; mild preservative odor |
| Quality Control | QC parameters per lot: particle size distribution (DLS, SEM), magnetic separation speed (kinetics measurement), DNA binding capacity, elution recovery efficiency, endotoxin (<0.1 EU/mg), DNase/RNase assay, sterility, pH, conductivity, and preservative concentration verification |
| Key Features | Ultra-fast magnetic separation (<5 seconds); optimized for high-throughput and automated processing; robust magnetic core enables use with weaker magnet configurations; reproducible performance in 96-well and 384-well formats; reduced incubation time compared to nanoparticles; compatible with magnet configurations in common liquid handler decks |
| Purity | >99% magnetite/silica composite; heavy metals (Pb, Cd, As, Hg) <10 ppm; endotoxin <0.1 EU/mg; DNase/RNase negative; bioburden <10 CFU/mL; residual TEOS and ammonia negative |
| Concentration | 20-50 mg/mL suspension; exact concentration specified per lot; dry weight per mL provided in certificate of analysis; particle count approximately 1-5 × 10^10 particles per mL at 20 mg/mL |
| Activity / Unit Definition | DNA binding capacity: 20-40 µg/mg (defined under standard salmon sperm DNA titration assay); binding capacity for large fragments (>5 kb) verified separately |
| Molecular Weight | Not applicable for particulate materials; each 500 nm bead contains approximately 10^7-10^8 superparamagnetic iron oxide nanocrystals embedded in amorphous silica matrix |
| Source / Origin | Wholly synthetic; iron oxide nanocrystals synthesized by thermal decomposition method; silica coating by controlled hydrolysis of tetraethyl orthosilicate; surface silanol groups generated by controlled hydration; no animal, plant, or biological-derived components |
| pH Range / Optimal pH | Stable suspension: pH 4.0-10.0; optimal DNA binding: pH 5.0-6.5; DNA elution: pH 8.0-9.0; isoelectric point approximately pH 2-3 (silica surface); irreversible aggregation at pH <3 or >11 |
| Shipping Conditions | Standard ambient temperature shipping; cold packs recommended for tropical/extreme heat destinations; protect from temperatures >40°C during transit; non-hazardous material; no special shipping documentation required |
| Expiration Date / Stability | 24 months from date of manufacture; confirmed by 24-month real-time stability program; accelerated stability: 45°C for 21 days equivalent to 24 months ambient; opened container stable for 6 months under sterile handling conditions; performance guaranteed through labeled expiry |
| Regulatory / Compliance | For research and OEM manufacturing use only; not for diagnostic procedures unless incorporated into a validated and registered IVD system; RoHS 3 compliant; heavy metals within directive limits; ISO 9001 certified manufacturing; REACH compliant for EU import |
| Compatibility | Fully compatible with laboratory automation platforms (Tecan, Hamilton, Beckman, PerkinElmer); works with all common binding buffer chemistries; eluted DNA suitable for PCR, qPCR, NGS, microarray; bead separation compatible with plate magnets, tube magnets, and custom magnet arrays |
| Recommended Buffer System | Stored in deionized water or phosphate buffer saline with antimicrobial preservative; binding requires high-salt chaotropic buffer; ethanol/isopropanol for washing; Tris-EDTA or water for elution; custom buffer systems evaluated upon request |
| Application Notes / Precautions | Vortex minimum 60 seconds before each use; beads settle rapidly—work quickly after vortexing or use tube rotator during processing; do not use if beads appear as hard sediment that does not resuspend; for difficult-to-pipette suspensions, dilute to 10 mg/mL working concentration; avoid using with filtered pipette tips that may retain beads; 500 nm beads are not suitable for applications requiring long-term suspension stability without agitation |
| Batch-to-Batch Consistency | DLS Z-average CV <12% batch-to-batch; PDI maintained <0.15 across all lots; DNA binding capacity CV <10%; magnetic separation time <5 seconds for all lots; iron content CV <8%; endotoxin <0.1 EU/mg every lot; comprehensive lot-specific certificate of analysis provided |
For research use only, not for clinical use.
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