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| Product Name | Silica-Coated Magnetic Beads for DNA Extraction (50 nm) |
| Catalog No. | MAGBEA-0001 |
| Description | Ultra-small 50 nm silica-coated superparamagnetic iron oxide nanoparticles optimized for rapid DNA binding and elution in nucleic acid extraction workflows. The high surface-area-to-volume ratio of the 50 nm particles provides exceptionally fast binding kinetics and high DNA loading capacity per unit mass. The thin, uniform silica coating ensures low non-specific binding of proteins and other cellular contaminants, yielding high-purity DNA suitable for sensitive downstream molecular biology applications. |
| Intended Use | Capture and purification of DNA fragments from enzymatic reactions, PCR products, agarose gel extracts, and cell lysates; component of custom DNA extraction kit formulations for developers of nucleic acid purification systems. |
| Principle / Technology | Superparamagnetic iron oxide (Fe3O4/γ-Fe2O3) core nanoparticles are coated with a thin, uniform layer of amorphous silica via a controlled sol-gel process. The silica surface provides silanol groups that bind DNA in the presence of high concentrations of chaotropic salts. DNA is released under low-ionic-strength, mildly alkaline conditions. The superparamagnetic core enables rapid magnetic separation without residual magnetism, allowing complete resuspension between steps. |
| Detection Method | Dynamic light scattering for particle size and polydispersity; transmission electron microscopy for core-shell morphology; vibrating sample magnetometry for saturation magnetization; UV spectrophotometry for DNA binding and elution efficiency; agarose gel electrophoresis for DNA integrity after purification |
| Sample Type | DNA in aqueous solution including PCR amplicons, restriction fragments, plasmid DNA, genomic DNA fragments, and cDNA; compatible with common binding buffers containing guanidine hydrochloride, guanidine isothiocyanate, or sodium iodide with ethanol or isopropanol |
| Performance Range / Specifications | Particle size: 50 ± 10 nm (DLS, Z-average); polydispersity index: <0.15; saturation magnetization: 30-50 emu/g; DNA binding capacity: 80-120 µg DNA per mg beads (for 200-1000 bp fragments); silica coating thickness: 5-10 nm; surface area: >60 m²/g; magnetic separation time: <10 seconds in standard magnetic rack |
| Sensitivity / LOD | Detectable DNA recovery from <1 ng input; effective for DNA purification from PCR reactions containing <10 pg/µL template; recovery >90% for DNA fragments 100 bp-5 kb |
| Specificity | Selective binding for double-stranded and single-stranded DNA; minimal RNA binding (<5% of total nucleic acid capacity) under optimized salt conditions; negligible protein binding with appropriate wash buffer composition; no detectable DNase or RNase activity |
| Reaction Conditions / Protocol | Equilibrate beads thoroughly before use by vortexing for 30 seconds; add 10-50 µL bead slurry (10-50 mg/mL) to DNA solution containing 1-5 volumes of binding buffer; incubate at room temperature for 5-10 minutes with gentle agitation; separate beads on magnetic rack for 1-2 minutes; remove supernatant; wash with 70-80% ethanol 2-3 times; air-dry beads briefly (do not over-dry); elute with 20-50 µL TE buffer or nuclease-free water at 56°C for 5 minutes; total procedure: 15-25 minutes |
| Components / Formulation | Silica-coated superparamagnetic iron oxide (Fe3O4) nanoparticles, 10-50 mg/mL in aqueous suspension (storage buffer: phosphate-buffered saline pH 7.4, 0.01-0.05% sodium azide preservative); particle concentration: 10^13-10^14 particles per mL depending on concentration |
| Storage Conditions | 2-8°C in tightly sealed container; do not freeze (freezing causes irreversible aggregation); protect from light; store upright to prevent cap contamination; vortex before each use to ensure homogeneous suspension |
| Shelf Life | 24 months from date of manufacture at recommended storage; performance verified at 0, 6, 12, 18, and 24 months; avoid repeated freeze-thaw cycles (stability of thawed product significantly reduced) |
| Package Specifications | 1 mL, 5 mL, and 10 mL suspensions at 10 mg/mL and 50 mg/mL; custom packaging available for OEM partners; supplied in low-protein-binding vials |
| Product Form | Dark brown to black aqueous suspension of superparamagnetic nanoparticles; homogeneous suspension after vortexing; free of visible aggregates |
| Quality Control | Each lot characterized by TEM (core size distribution, coating uniformity), DLS (hydrodynamic size, PDI), DNA binding capacity (salmon sperm DNA titration), elution efficiency, saturation magnetization (VSM), absence of DNase/RNase, and endotoxin level; certificate of analysis provided |
| Key Features | Ultra-small 50 nm particle size for maximal surface area and rapid kinetics; thin uniform silica coating for low non-specific binding; superparamagnetic for complete resuspension; stable colloidal suspension with minimal sedimentation; compatible with standard magnetic separation racks; high DNA capacity per unit mass |
| Purity | >99% magnetite/silica composite; endotoxin <0.1 EU/mg beads; DNase/RNase free; heavy metal content (Pb, Cd, Hg, As) <10 ppm each |
| Concentration | 10 mg/mL or 50 mg/mL in aqueous suspension; particle number concentration available upon request; A280 and Bradford protein assays show no detectable protein contamination |
| Activity / Unit Definition | DNA binding capacity: 80-120 µg/mg (defined as mass of λ DNA HindIII digest bound per mg dry bead weight under standard binding conditions) |
| Molecular Weight | Not applicable for nanoparticulate materials; density approximately 2.5-3.5 g/cm³ for silica-coated magnetite nanoparticles |
| Source / Origin | Synthetic; iron oxide core produced by co-precipitation of Fe²+ and Fe³+ salts under alkaline conditions; silica coating applied via tetraethyl orthosilicate (TEOS) sol-gel hydrolysis-condensation; no biological source materials |
| pH Range / Optimal pH | Stable colloidal suspension: pH 6.0-9.0; optimal DNA binding: pH 5.0-6.5; optimal DNA elution: pH 8.0-9.0; irreversible aggregation occurs below pH 4.0 or above pH 10.0 |
| Shipping Conditions | Ambient temperature acceptable (<30°C); cold packs recommended for summer shipping to prevent excessive heating; protect from freezing during winter transit; classified as non-hazardous for transport |
| Expiration Date / Stability | 24 months at 2-8°C; accelerated stability at 37°C for 30 days confirms performance maintenance; freeze-thaw causes irreversible aggregation—verify product condition upon receipt; preservative (sodium azide) effective for 24 months; after opening, use within 6 months under sterile technique |
| Regulatory / Compliance | For research and industrial use only; not for diagnostic or therapeutic applications; non-hazardous for transport; SDS available; manufactured under ISO 9001 quality management |
| Compatibility | Compatible with guanidine hydrochloride, guanidine isothiocyanate, and sodium iodide binding buffers; compatible with ethanol and isopropanol precipitation aids; works with all standard magnetic separation racks (neodymium magnet-based); eluted DNA suitable for PCR, qPCR, NGS, cloning |
| Recommended Buffer System | Supplied in PBS pH 7.4 with 0.01-0.05% sodium azide; binding requires chaotropic salt buffer (user-supplied or custom-formulated); elution in 10 mM Tris-HCl pH 8.0-8.5 or nuclease-free water |
| Application Notes / Precautions | Always vortex bead suspension vigorously before pipetting to ensure homogeneous slurry; use positive displacement or wide-bore pipette tips for viscous 50 mg/mL suspensions; avoid contact of bead suspension with strong acids, bases, or oxidizing agents; do not sonicate at high power (causes silica coating delamination); for custom buffer compatibility testing, contact technical support; sodium azide may interfere with certain enzymatic reactions—thorough washing recommended |
| Batch-to-Batch Consistency | Particle size CV <15% between lots (DLS Z-average); DNA binding capacity CV <12% between lots; saturation magnetization CV <10%; silica coating integrity verified per lot by TEM; endotoxin <0.1 EU/mg per lot; DNase/RNase tested per batch; each lot provided with lot-specific characterization certificate |
For research use only, not for clinical use.
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