Mouse CD8+ Cell Isolation Kit (Positive Selection)
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Mouse CD8+ Cell Isolation Kit (Positive Selection)

Cat.No: SM-HMM-0045 Datasheet

Specification Quantities

1×10^8 cells:
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1×10^9 cells:
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Product Details Product Overview Related Products
Product Name Mouse CD8+ Cell Isolation Kit (Positive Selection)
Catalog No. SM-HMM-0045
Description The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field. Our Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions.
Features Purity of 95% or more.
Wide range of sample applicability for direct capture of target cells.
Magnetic bead release technology cells are not labeled with magnetic beads.
Storage Store at 2-8°C, do not freeze.
Applications This kit is suitable for sorting CD8+ cells from mouse lymphoid organs such as spleen and lymph nodes.
Product Introduction This kit employs a direct positive selection strategy using magnetic beads conjugated to an antibody specific for mouse CD8a. It is designed for the rapid and efficient isolation of CD8+ T cells where maximum cell recovery is prioritized, and the presence of bead-bound antibody on the cell surface is not detrimental to downstream assays. The simple, one-step incubation and magnetic separation process yields a highly enriched population suitable for applications like RNA/DNA extraction, bulk sequencing, or when starting cell numbers are limited.
Product Application • High-recovery isolation of CD8+ T cells for genomic or transcriptomic analysis (RNA-seq, qPCR)
• Rapid enrichment of CD8+ cells from limited starting material
• Preparation of cells where antibody coating is not a concern (e.g., certain types of fixed-cell analysis)
• Simplified protocol for teaching or high-throughput screening contexts.
Product Advantages • Maximized Recovery: The direct positive selection approach typically yields higher recovery rates of the target population compared to negative selection, crucial when working with rare samples.
• Speed & Simplicity: Involves a single labeling and separation step, offering one of the fastest paths to an enriched CD8+ population.
• High Purity: Effectively enriches CD8+ T cells to high purity levels, sufficient for many molecular and cellular analyses.
• Ideal for Molecular Studies: The isolated cells are excellent for applications where cell function is not the primary endpoint, such as gene expression profiling.

For research use only, not for clinical use.

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