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| Product Name | Fluorescent Magnetic Microspheres for Lateral Flow Assays (300 nm, Red) |
| Catalog No. | MAGBEA-0022 |
| Description | Superparamagnetic fluorescent microspheres internally doped with red fluorescent dye and coated with carboxyl surface groups for covalent antibody conjugation. The dual magnetic and fluorescent properties enable both magnetic separation during conjugation and washing steps and fluorescent signal detection in lateral flow test strips. These particles are designed for high-sensitivity sandwich immunoassay development in rapid diagnostic test platforms. |
| Intended Use | Fluorescent labeling and magnetic capture platform for lateral flow immunoassay test strip development. |
| Principle / Technology | Fluorescent dye-embedded polymer microspheres with surface carboxyl groups for antibody conjugation and superparamagnetic core for separation |
| Detection Method | Fluorescence reader for lateral flow test strips; UV lamp for visual confirmation |
| Sample Type | Lateral flow immunoassay test strips for infectious disease, cardiac marker, and hormone detection |
| Performance Range / Specifications | Particle diameter: 300 nm; excitation maximum approximately 540 nm; emission maximum approximately 610 nm; carboxyl group density: ≥0.5 mmol/g |
| Sensitivity / LOD | Fluorescence signal detectable from 0.1 ng of conjugated antibody per test line |
| Specificity | Surface carboxyl groups for standard EDC/NHS antibody conjugation chemistry |
| Reaction Conditions / Protocol | Activate carboxyl groups with EDC/NHS 15 min at room temperature; couple antibody in MES buffer pH 6.0 for 2 hr; block with BSA 30 min; wash and resuspend in storage buffer |
| Components / Formulation | Fluorescent magnetic microsphere suspension (1% solids in water), EDC crosslinker (lyophilized), NHS (lyophilized), MES activation buffer, storage buffer |
| Storage Conditions | Beads: 2–8 °C, protected from light; EDC and NHS: –20 °C desiccated |
| Shelf Life | 12 months for beads; 24 months for EDC/NHS at –20 °C |
| Package Specifications | 1 mL bead suspension |
| Product Form | Aqueous bead suspension, red fluorescent |
| Quality Control | Fluorescence quantum yield and magnetic separation time verified per lot; conjugation efficiency tested with mouse IgG standard |
| Key Features | Dual magnetic and fluorescent functionality streamlines washing and detection without separate magnetic and fluorescent particle preparations |
| Purity | Endotoxin ≤0.1 EU/mg; heavy metal content ≤10 ppm |
| Concentration | As specified (mg/mL suspension) |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Synthetic polymer-magnetite composite; recombinant protein ligands where applicable |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Ambient temperature; protect from freezing for aqueous suspensions |
| Expiration Date / Stability | 18–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; ISO 9001 manufacturing; custom GMP options available |
| Compatibility | Compatible with manual magnetic racks and automated KingFisher and similar platforms |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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