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| Product Name | Silica-Coated Magnetic Beads for DNA Extraction (200 nm) |
| Catalog No. | MAGBEA-0002 |
| Description | Silica-coated superparamagnetic beads with an average diameter of 200 nm, offering an optimal balance between sedimentation stability and magnetic separation speed for routine DNA purification applications. The engineered silica surface chemistry provides high and reproducible DNA binding capacity across a wide range of fragment sizes, from short PCR products to high-molecular-weight genomic DNA. The 200 nm size class enables rapid magnetic separation while maintaining adequate surface area for efficient nucleic acid capture. |
| Intended Use | General-purpose DNA purification from aqueous solutions in research and OEM nucleic acid extraction system development; suitable for genomic DNA, plasmid DNA, PCR products, and enzymatic reaction cleanup across manual and automated liquid handling platforms. |
| Principle / Technology | Superparamagnetic iron oxide cores are coated with a controlled-thickness silica shell through a Stöber process modification. The silica surface presents silanol (Si-OH) groups that interact with DNA through hydrogen bonding and salt bridge formation in the presence of chaotropic agents at acidic to neutral pH. DNA release occurs by disrupting these interactions under low-salt, mildly alkaline conditions. The superparamagnetic property ensures rapid magnetic collection and complete redispersion. |
| Detection Method | Dynamic light scattering and laser diffraction for particle size analysis; scanning and transmission electron microscopy for morphology; magnetometry for magnetic properties; DNA binding isotherm analysis; agarose gel electrophoresis for purified DNA quality; PCR functional testing |
| Sample Type | DNA samples including genomic DNA, plasmid DNA, PCR products, restriction fragments, cDNA, and fragmented DNA for NGS library preparation; works with binding systems based on guanidine hydrochloride, guanidine isothiocyanate, sodium perchlorate, or sodium iodide |
| Performance Range / Specifications | Particle diameter: 200 ± 30 nm (DLS); PDI: <0.20; saturation magnetization: 40-55 emu/g; DNA binding capacity: 40-80 µg DNA per mg beads (for 500 bp DNA fragment); magnetic separation time: <30 seconds on standard rack; surface area: 15-25 m²/g; solids content: 10-50 mg/mL in suspension |
| Sensitivity / LOD | Quantitative DNA recovery from solutions containing as low as 10 ng/mL DNA; effective purification of PCR products with <50 ng total yield; recovery >85% for DNA across 100 bp-10 kb range |
| Specificity | High affinity for double-stranded and single-stranded DNA; RNA binding <10% under standard binding conditions (can be modulated by ethanol concentration); protein carryover <1% with appropriate wash protocols; no PCR inhibitor carryover when using recommended wash buffer |
| Reaction Conditions / Protocol | Vortex bead vial thoroughly for 60 seconds; transfer desired volume (typically 10-50 µL of 20 mg/mL suspension) to sample containing DNA and binding buffer; incubate 5-10 minutes at room temperature with gentle shaking; place on magnetic rack for 1-2 minutes; discard supernatant; wash beads twice with 70% ethanol (500 µL each); briefly air-dry for 2-5 minutes; elute DNA in pre-warmed (56°C) TE buffer or nuclease-free water; incubate 5 minutes; separate beads; collect supernatant; total duration: approximately 20 minutes |
| Components / Formulation | Silica-coated superparamagnetic iron oxide beads, 20-50 mg/mL suspension in deionized water or phosphate buffer with 0.02% sodium azide preservative; beads per mg: approximately 10^10 particles |
| Storage Conditions | 2-8°C in original container tightly sealed; store upright; do not freeze; protect from light; vortex thoroughly before each use; use aseptic technique to prevent microbial contamination |
| Shelf Life | 24 months at 2-8°C; performance monitoring at 0, 12, and 24 months; accelerated stability data (37°C, 30 days) supports shelf life; prevention of microbial contamination is critical for long-term stability |
| Package Specifications | 1 mL, 5 mL, 10 mL, 50 mL, and 100 mL suspensions; concentrations of 10, 20, and 50 mg/mL; bulk OEM packaging available; supplied in high-density polyethylene or glass bottles |
| Product Form | Dark brown to black aqueous suspension; free-flowing after vortexing; no visible settling after 30 minutes in diluted working suspension; occasional soft sediment that resuspends completely |
| Quality Control | Lot release testing: particle size by DLS and SEM, DNA binding capacity, elution efficiency, magnetic separation time, endotoxin, DNase/RNase, sterility (bioburden <10 CFU/mL), pH, conductivity; certificate of analysis with quantitative results |
| Key Features | Balanced 200 nm size for rapid separation and good suspension stability; high reproducibility across manufacturing lots; broad DNA size compatibility; compatible with automated liquid handlers; ready-to-use aqueous suspension; cost-effective for high-throughput applications |
| Purity | >98% magnetite/silica; endotoxin <0.05 EU/mg; DNase and RNase free; transition metal ions <5 ppm each; residual synthesis reagents (TEOS, ammonia) below detection limit |
| Concentration | 10-50 mg/mL bead concentration (specified on lot label); absorbance at 500 nm (1:100 dilution) used for lot-to-lot consistency monitoring; total solids by gravimetric analysis provided per lot |
| Activity / Unit Definition | DNA binding capacity: 40-80 µg/mg (salmon sperm DNA in guanidine hydrochloride binding buffer, measured by UV absorbance difference); activity retained >90% after 24 months storage |
| Molecular Weight | Not applicable for particulate materials; iron oxide core molecular weight approximately 231.5 g/mol (Fe3O4); silica shell is amorphous SiO2 (60.1 g/mol per SiO2 unit) |
| Source / Origin | Synthetic; magnetite cores by controlled co-precipitation method; silica coating by modified Stöber process using tetraethyl orthosilicate in alkaline ethanol-water mixture; all raw materials are chemically synthesized; no animal, plant, or biological source materials |
| pH Range / Optimal pH | Stable in pH 4.5-10.0 as suspension; optimal DNA binding pH 5.0-6.5 (chaotropic salt system); optimal elution pH 7.5-9.0; isoelectric point of silica surface approximately pH 2-3 |
| Shipping Conditions | Ambient temperature acceptable; cold packs recommended for shipments during extreme heat; protect from freezing; classified as non-hazardous; UN number not applicable |
| Expiration Date / Stability | 24 months at 2-8°C confirmed by real-time monitoring of DNA binding capacity, colloidal stability, and magnetic responsiveness; accelerated aging at 40°C/75% RH for 4 weeks shows <10% performance variation; microbial preservative maintains efficacy throughout shelf life |
| Regulatory / Compliance | For research, development, and OEM manufacturing use; not for clinical diagnostic or therapeutic use without appropriate regulatory clearance; RoHS compliant; Certificate of Analysis per lot; ISO 9001:2015 certified manufacturing |
| Compatibility | Compatible with all major commercial DNA binding buffers (guanidine-based and sodium iodide-based); compatible with ethanol and isopropanol; suitable for use in Tecan, Hamilton, Beckman Coulter, KingFisher, and custom automated extraction platforms; eluted DNA suitable for all downstream molecular biology applications |
| Recommended Buffer System | Supplied in deionized water or PBS with sodium azide preservative; DNA binding requires chaotropic salt buffer (not supplied); recommended elution buffer: 10 mM Tris-HCl pH 8.0-8.5, 0.1 mM EDTA or nuclease-free water |
| Application Notes / Precautions | Vortex bead suspension for at least 60 seconds before each use until completely homogeneous; use within 30 minutes of aliquoting for best reproducibility; minimize exposure of opened container to air to reduce oxidation risk; for viscous samples, increase bead incubation time; if beads settle during storage, vortexing restores full suspension without performance loss; bead aggregation can occur if exposed to high salt without DNA—always add DNA to binding buffer before beads |
| Batch-to-Batch Consistency | Particle size (DLS Z-average) CV <15% between lots; DNA binding capacity CV <10%; magnetic separation time (T50) CV <8%; iron content by ICP-OES CV <5%; endotoxin consistently <0.05 EU/mg across all lots; comprehensive lot-to-lot characterization available |
For research use only, not for clinical use.
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