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| Product Name | Serum Iron Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0006 |
| Description | Iron is one of the essential trace elements in human body, and is also the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems. It plays an important role in oxygen transport and fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders and affect the immune function of the body. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 4 h (100 Samples) |
| Storage | Store at 4°C away from light |
| Self-contained Reagents | CHCl3，MW=119.39，CAS: 67-66-3; C2H4O2，MW=60.05，CAS: 64-19-7 |
| Detection Principle | Sodium sulfite can reduce Fe3+ to generate Fe2+, Fe2+ further color development with 2,2'-bipyridine, the product has a characteristic absorption peak at 520 nm, through the absorbance value can be quantitatively detected by the change of serum iron content. |
| Detection Methods | 2,2'-Bipyridine Colorimetric Method |
| Detection Wavelength | 520 nm |
| Signal Response | Incremental |
| Standard | Iron Standard Solution |
| Reference Standards | y=1.716x+0.0016 (R2=0.9999) |
| Standard Linear Range | 0.025-0.3 µmol/mL |
| Detection Limit | 0.002 µmol/mL |
| Note | If the absorbance value exceeds the linear range of the standard absorbance value: higher than the highest value, it is recommended to dilute the serum (plasma) to be tested appropriately before measurement, lower than the lowest value, it is recommended to increase the sample volume appropriately before measurement, and the calculation is modified accordingly; the validity period of reagent 2 is relatively short after the preparation of reagent 2, in order to facilitate the experimental arrangements, a bottle is attached as a spare, each bottle can meet the determination of at least 60 samples. |
Iron is an indispensable trace element for various biological processes in the human body, serving as a core component of key biomolecules. Beyond hemoglobin and myoglobin— which are critical for oxygen transport in the bloodstream and muscle tissue— iron is also a cofactor for numerous enzymes involved in energy metabolism, DNA synthesis, and antioxidant defense mechanisms. These enzymes include cytochrome c oxidase, which plays a vital role in cellular respiration, and catalase, which protects cells from oxidative damage.
The accurate measurement of serum iron content is essential in research settings to investigate iron metabolism disorders, nutritional deficiencies, and their links to various physiological and pathological conditions. For instance, research on iron deficiency often focuses on understanding its impact on hematopoietic function, immune response modulation, and even neurodevelopment in model organisms. Conversely, studies on iron overload explore its association with oxidative stress and organ damage.
Traditional methods for iron detection often involve complex sample preparation, long assay times, or low sensitivity, which can limit research efficiency. The Serum Iron Content Assay Kit (Microplate Reader) addresses these challenges by leveraging a reliable colorimetric method, enabling researchers to obtain precise and reproducible results for large sample sets— a key requirement in high-throughput research environments.
This assay kit is designed exclusively for research applications, supporting studies in fields such as biochemistry, physiology, nutrition science, and pharmacology. It provides a standardized tool for researchers to quantify serum iron levels, facilitating data comparison across experiments and laboratories.
Utilizes the 2,2'-Bipyridine Colorimetric Method, a well-established and widely recognized technique for iron quantification, ensuring consistency with conventional research protocols.
Compatible with microplate readers, enabling high-throughput analysis that saves time and labor when processing multiple samples simultaneously.
Includes a matched 96-well plate, eliminating the need for additional equipment or consumable purchases and simplifying experimental setup.
Offers a wide standard linear range of 0.025-0.3 µmol/mL, covering the typical serum iron concentration range in most research models and reducing the need for excessive sample dilution or concentration.
Boasts a low detection limit of 0.002 µmol/mL, ensuring high sensitivity to detect even trace amounts of iron in samples with low iron content.
Provides a clear incremental signal response at 520 nm, allowing for easy and accurate absorbance reading and quantitative analysis.
Comes with pre-formulated self-contained reagents (including CHCl3 and C2H4O2) and a spare reagent bottle for Reagent 2, ensuring sufficient supply for at least 60 samples per bottle and supporting flexible experimental scheduling.
Requires a reasonable estimated measurement time of 4 hours for 100 samples, balancing efficiency with assay accuracy.
Stores at 4°C away from light, offering convenient storage conditions without the need for ultra-low temperature freezers.
High accuracy and reliability, supported by a reference standard curve with an R² value of 0.9999, ensuring minimal deviation and trustworthy quantitative results.
User-friendly design with clear operating guidelines, making it suitable for both experienced researchers and laboratory technicians with basic assay skills.
Cost-effective solution, as the kit provides sufficient reagents for 100 testable samples, reducing per-sample analysis costs compared to single-use or low-throughput alternatives.
Flexible sample handling: allows for appropriate dilution of samples with absorbance values exceeding the standard linear range or increased sample volume for samples below the range, ensuring adaptability to diverse sample types.
Consistent performance across experiments, thanks to standardized reagent formulations and strict quality control during production, minimizing batch-to-batch variation.
Focused on research applicability, with specifications tailored to the needs of academic, pharmaceutical, and biotechnological research, avoiding unnecessary clinical-related features that add complexity.
Time-efficient workflow that integrates sample processing and detection into a streamlined protocol, enabling researchers to allocate more time to data analysis and interpretation.
For research use only, not for clinical use.
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