| Product Name | High Density Lipoprotein Cholesterol (HDL-C) Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0022 |
| Description | High-density lipoprotein (HDL), as an anti-atherosclerotic lipoprotein, can transport cholesterol from extrahepatic tissues to the liver for metabolism, converting it into bile acids or directly excreting it from the intestine through bile, thereby reducing cholesterol deposition on the arterial walls. The anti-atherosclerotic effect is achieved through various means such as promoting reverse cholesterol transport, anti-inflammation, anti-oxidation, inhibiting thrombosis formation and improving endothelial cell function. The level of its content in the blood is of great significance for the risk assessment of cardiovascular diseases. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 6 h (100 Samples) |
| Storage | Store at 4°C away from light |
| Self-contained Reagents | / |
| Detection Principle | The CHOD-PAP endpoint method combined with the classical GPO Trinder enzymatic reaction was used for the determination. Cholesterol esterase (CHE) was able to break down cholesterol esters into free cholesterol, and cholesterol oxidase (COD) further oxidized free cholesterol into cholestenone and H2O2, and H2O2 was able to react with 4-aminoantipyrine to produce red benzoquinone imine catalyzed by peroxidase (POD). H2O2 can be catalyzed by peroxidase (POD) to react with 4-aminoantipyrine to form red benzoquinone imine, and the product has a characteristic absorption peak at 550 nm, which can be used to quantitatively detect the HDL cholesterol content by the change of absorbance value. |
| Detection Methods | CHOD-PAP End Point Method |
| Detection Wavelength | 550 nm |
| Signal Response | Incremental |
| Standard | Cholesterol |
| Reference Standards | y=0.391x-0.0037 (R2=0.9992) |
| Standard Linear Range | 0.125-3.0 mmol/L |
| Detection Limit | 0.125 mmol/L |
| Note | If the A measurement or ΔA measurement exceeds the standard linear absorbance range: higher than the maximum value, it is recommended to appropriately dilute the sample to be tested with PBS or normal saline before conducting the measurement. If the sample size is lower than the minimum value, it is recommended to appropriately increase the sample size before conducting the measurement and make corresponding modifications during the calculation. This product adopts the HDL separation method and cholesterol determination method. It is simple and reliable to operate, has high sensitivity and good repeatability, and is not affected by the chemical substances in the vast majority of samples. However, in the system, vitamin C>0.18 g/L, hemoglobin >2 g/L, bilirubin >0.25 g/L, and strong reducing agents (such as dithiostachitol, mercaptoethanol, etc.) will interfere with the test results; Naturally coagulated serum and EDTA anticoagulated plasma can be used. Heparin is not recommended as an anticoagulant. |
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