| Product Name | Angiotensin Converting Enzyme (ACE) Activity Assay Kit (UV Spectrophotometer) |
| Catalog No. | BBITK-HMM-0031 |
| Description | Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxyl peptidase, mainly existing in endothelial cells of various tissues such as the lungs, brain, and kidneys. Its main function is to inactivate bradykinin and catalyze the conversion of angiotensin I to angiotensin II, playing an important regulatory role in the angiotensin system. And it is closely related to physiological processes such as blood pressure, cardiovascular function and water-salt balance. |
| Testing Equipment | UV Spectrophotometer |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 4 h (48 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Angiotensin-converting enzyme catalyzes the hydrolysis of the substrate N-[3-(2-furyl)acryloyl]-L-phenylalanyl-glycyl-glycyl-glycine (FAPGG) to produce furanylacryloyl-L-phenylalanine (FAP) and bis-glycyl-glycyl-glycine peptide (GG), and FAPGG has a characteristic peak at 340 nm, which is characterized by a change of absorbance value. |
| Detection Methods | FAPGG Method |
| Detection Wavelength | 340 nm |
| Signal Response | Decremental |
| Note | Accurately complete the absorbance measurement at the specified time points to ensure the accuracy and reproducibility of the experimental results; if A1 is greater than 1.2 or ΔA is greater than 0.4, it is recommended that the crude enzyme solution be appropriately diluted with Reagent I before measurement; if ΔA is less than 0.02, it is recommended that the enzyme reaction time be appropriately prolonged or the sample volume be increased before measurement, and the calculations should be modified accordingly. |
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